Evidence map›Paper›PMID 35881357›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Protein Microarrays as a Tool to Analyze Antibody Responses to Variant Surface Antigens Expressed on the Surface of Plasmodium falciparum-Infected Erythrocytes.

Albert E Zhou, Aarti Jain, Rie Nakajima, Biraj Shrestha, Emily M Stucke, Sudhaunshu Joshi, Kathy A Strauss, Per N Hedde, Andrea A Berry, Philip L Felgner and 1 more

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
14.3field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

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  4. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Albert E ZhouMalaria Research Program, Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, MD, USA.ORCID 0000-0003-1126-9825
Aarti JainVaccine R&D Center, Department of Physiology and Biophysics, University of California Irvine, Irvine, CA, USA.
Rie NakajimaVaccine R&D Center, Department of Physiology and Biophysics, University of California Irvine, Irvine, CA, USA.
Biraj ShresthaMalaria Research Program, Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, MD, USA.
Emily M StuckeMalaria Research Program, Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, MD, USA.
Sudhaunshu JoshiMalaria Research Program, Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, MD, USA.
Kathy A StraussMalaria Research Program, Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, MD, USA.
Per N HeddeDepartment of Pharmaceutical Sciences, University of California Irvine, Irvine, CA, USA.
Andrea A BerryMalaria Research Program, Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, MD, USA.ORCID 0000-0003-3140-3868
Philip L FelgnerVaccine R&D Center, Department of Physiology and Biophysics, University of California Irvine, Irvine, CA, USA.ORCID 0000-0002-4117-8505
Mark A TravassosMalaria Research Program, Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, MD, USA. mtravass@som.umaryland.edu.ORCID 0000-0002-6045-3322
University of Maryland, Baltimore · USUniversity of California, Irvine · US

Funding

Malaria variant surface antigen expression and vulnerability to disease in Malian children with Hemoglobin C traitR01HL146377 · NHLBI · UNIVERSITY OF MARYLAND BALTIMORE · PI TRAVASSOS, MARK A · 2019 to 2023
$1.9M
6 · The paper itself

Abstract

Enzyme-linked immunosorbent assays (ELISAs) remain the gold standard for measuring antibodies, but are time-consuming and use significant amounts of precious sample and reagents. Protein microarrays represent an appealing alternative, particularly for studies focused on large gene families such as those encoding variant surface antigens in the malaria parasite Plasmodium falciparum. Such microarrays represent an ideal high-throughput platform to study antibody responses to hundreds of malaria parasite variant surface antigens at once, providing critical insights into the development of natural immunity to malaria. We describe the essential background and approach to run an assay using a P. falciparum microarray populated with variant surface antigens. This allows the user to define serologic profiles and identify serodominant antigens that represent promising targets for vaccine or therapeutic development.

Indexed as

MalariaMalaria, FalciparumAntibodies, ProtozoanAntibody FormationAntigens, ProtozoanAntigens, SurfaceErythrocytesHumansPlasmodium falciparumProtein Array AnalysisProtozoan ProteinsAntibodies, ProtozoanAntigens, ProtozoanAntigens, SurfaceProtozoan ProteinsAntibodiesBiomarkerImmunityImmunofluorescenceMalariaPlasmodium falciparumProteinProtein microarraySerology

Identifiers

PMID35881357
OpenAlexW4288062570

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.