Evidence map›Paper›PMID 35881356›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

High-Throughput BioPlex Assay for the Study of Functionally Active Plasmodium Falciparum Antigens That Are Expressed on the Surface of Infected Erythrocytes.

Andrew V Oleinikov

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 68% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author at 1 institution in 1 country.

Andrew V OleinikovCharles E. Schmidt College of Medicine, Florida Atlantic University, Boca Raton, FL, USA. aoleinikov@health.fau.edu.
Florida Atlantic University · US

Funding

Identification of vaccine candidates against severe malariaR01AI092120 · NIAID · SEATTLE BIOMEDICAL RESEARCH INSTITUTE · PI OLEINIKOV, ANDREW V · 2011 to 2014
$1.6M
High Throughput Analysis of Malarial Surface AntigensR21AI064503 · NIAID · SEATTLE BIOMEDICAL RESEARCH INSTITUTE · PI OLEINIKOV, ANDREW V · 2007 to 2008
$503k
High throughput screening for anti-adhesion drugs against placental and cerebral malariaR21AI137721 · NIAID · FLORIDA ATLANTIC UNIVERSITY · PI NEFZI, ADEL, OLEINIKOV, ANDREW V · 2018 to 2019
$476k
High throughput screening for anti-adhesion drugs to treat severe malariaR56AI083668 · NIAID · SEATTLE BIOMEDICAL RESEARCH INSTITUTE · PI OLEINIKOV, ANDREW V · 2010 to 2010
$433k
NIAID NIH HHS R01 AI092120NIAID NIH HHS R21 AI064503NIAID NIH HHS R21 AI137721NIAID NIH HHS R56 AI083668
6 · The paper itself

Abstract

Identification of P. falciparum infected erythrocyte surface ligands (such as PfEMP1) matched with the host receptors they interact with, as well as identification of PfEMP1 domains that are targets of protective immunity, are important for understanding of the pathophysiology of severe malaria (SM) and for design of novel vaccine candidates. In addition, identification of small-molecule drugs that can prevent or reverse receptor-ligand domain interactions could provide new tools for adjunctive therapy in SM. This protocol describes how to prepare functionally intact PfEMP1 proteins in mammalian cells (COS-7) and immobilize them on the surface of BioPlex beads. Furthermore, the protocol described how to identify PfEMP1 constructs that bind to specific host receptors or to immunoglobulins (IgG, IgM, etc.), and how to measure inhibition of the receptor binding to PfEMP1 constructs by small-molecule compounds or serum/plasma.

Indexed as

Malaria, FalciparumPlasmodium falciparumAnimalsAntibodies, ProtozoanAntigens, ProtozoanErythrocytesHumansLigandsMammalsPolymersProtozoan ProteinsAntibodies, ProtozoanAntigens, ProtozoanbioplexLigandsPolymersProtozoan ProteinsAnti-adhesion antibodyAnti-adhesion drugsAnti-PfEMP1 immune responseCytoadhesionHigh throughput analysisHost receptor: PfEMP1 interactionsLibrary of functional PfEMP1 constructsMammalian protein expression

Identifiers

PMID35881356
OpenAlexW4288062305

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.