ArticleThe Plant cell2022
The mycorrhiza-specific ammonium transporter ZmAMT3;1 mediates mycorrhiza-dependent nitrogen uptake in maize roots.
Article in The Plant cell, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed, 100 citations in OpenAlex.
- Automated Phenotyping Unveils Trait-Specific Genotypic Variation in Arbuscular Mycorrhiza Responsiveness in Maize (Zea mays).Plant, cell & environment · 2026Article
- Integrating single-cell omic techniques to resolve the spatio-temporal complexity of arbuscular mycorrhizal symbiosis.Journal of experimental botany · 2026Review
- Review
- Achieving High-Density and Stress-Resilient Maize Breeding Via Germplasm Innovation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Review
- Physiological and Molecular Mechanisms of Nitrogen Regulation on Grain Quality in Cereal Crops at Later Stages.International journal of molecular sciences · 2026Review
- VviAMT4;1 Is a High-Affinity Ammonium Transporter in Table Grape.Plants (Basel, Switzerland) · 2026Article
- Deciphering the nitrogen-promoting effect of vermicompost: from a complex organic matrix to synthetic microbial communities.Frontiers in plant science · 2026Review
- A Whole-Plant Culture Method to Study Structural and Functional Traits of Extraradical Mycelium.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Genome-Wide Identification of theGenes · 2025Article
- Decades' progress and prospects on maize functional genomics and molecular breeding.Science China. Life sciences · 2025Review
- Effects of AM fungal inoculation on the growth, nitrogen uptake and metabolism of Camellia Oleifera under different nitrogen levels.BMC plant biology · 2025Article
- Arbuscular mycorrhizal fungi mitigated nitrogen leaching by enhancing soil nitrogen retention inApplied and environmental microbiology · 2025Article
- Functional Characterization of Ammonium TransporterJournal of fungi (Basel, Switzerland) · 2025Article
- OsNLP3 and OsPHR2 orchestrate direct and mycorrhizal pathways for nitrate uptake by regulating NAR2.1-NRT2s complexes in rice.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- The Mechanism of Ammonia-Assimilating Bacteria Promoting the Growth of Oyster Mushrooms (Journal of fungi (Basel, Switzerland) · 2025Article
- Optimizing nitrogen use efficiency in forest plantations: mechanistic insights fromForestry research · 2025Review
- Genotype-specific responses of maize plants toFrontiers in plant science · 2025Article
- AMF promotes the structure and metabolic activity of rhizosphere soil microbial communities in areca/vanilla intercropping system under nitrogen-limited conditions.Frontiers in microbiology · 2025Article
- Review
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Authors and funding
12 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most plant species can form symbioses with arbuscular mycorrhizal fungi (AMFs), which may enhance the host plant's acquisition of soil nutrients. In contrast to phosphorus nutrition, the molecular mechanism of mycorrhizal nitrogen (N) uptake remains largely unknown, and its physiological relevance is unclear. Here, we identified a gene encoding an AMF-inducible ammonium transporter, ZmAMT3;1, in maize (Zea mays) roots. ZmAMT3;1 was specifically expressed in arbuscule-containing cortical cells and the encoded protein was localized at the peri-arbuscular membrane. Functional analysis in yeast and Xenopus oocytes indicated that ZmAMT3;1 mediated high-affinity ammonium transport, with the substrate NH4+ being accessed, but likely translocating uncharged NH3. Phosphorylation of ZmAMT3;1 at the C-terminus suppressed transport activity. Using ZmAMT3;1-RNAi transgenic maize lines grown in compartmented pot experiments, we demonstrated that substantial quantities of N were transferred from AMF to plants, and 68%-74% of this capacity was conferred by ZmAMT3;1. Under field conditions, the ZmAMT3;1-dependent mycorrhizal N pathway contributed >30% of postsilking N uptake. Furthermore, AMFs downregulated ZmAMT1;1a and ZmAMT1;3 protein abundance and transport activities expressed in the root epidermis, suggesting a trade-off between mycorrhizal and direct root N-uptake pathways. Taken together, our results provide a comprehensive understanding of mycorrhiza-dependent N uptake in maize and present a promising approach to improve N-acquisition efficiency via plant-microbe interactions.
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