ArticleJournal of the American Chemical Society2022
Template-Free Assembly of Functional RNAs by Loop-Closing Ligation.
Article in Journal of the American Chemical Society, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Influence of Phosphate Activation Chemistry on the Selection of the Primordial Genetic Alphabet.Journal of the American Chemical Society · 2026Article
- Crystal structure of a tRNA acceptor-stem mimic at 1.94 Å resolution.Acta crystallographica. Section F, Structural biology communications · 2026Article
- Emergent Chemical Reactivity and Complexity of RNA Condensates.Angewandte Chemie (International ed. in English) · 2025Article
- Functions and applications of enzymes in nucleic acid nanotechnology.Journal of nanobiotechnology · 2025Review
- Efficient Assembly of Functional RNA byJournal of the American Chemical Society · 2025Article
- Origin of the RNA World in Cold Hadean Geothermal Fields Enriched in Zinc and Potassium: Abiogenesis as a Positive Fallout from the Moon-Forming Impact?Life (Basel, Switzerland) · 2025Article
- Selective Nonenzymatic Formation of Biologically Common RNA Hairpins.Angewandte Chemie (International ed. in English) · 2025Article
- Selection-diversification interplay in oligonucleotide chemical evolution.Biophysics and physicobiology · 2025Article
- A potential role for RNA aminoacylation prior to its role in peptide synthesis.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Prebiotic synthesis of dihydrouridine by photoreduction of uridine in formamide.Chemical communications (Cambridge, England) · 2024Article
- Manganese(II) promotes prebiotically plausible non-enzymatic RNA ligation reactions.Chemical communications (Cambridge, England) · 2024Article
- On the origin of life: an RNA-focused synthesis and narrative.RNA (New York, N.Y.) · 2023Article
- Small-Molecule Organocatalysis Facilitates In Situ Nucleotide Activation and RNA Copying.Journal of the American Chemical Society · 2023Article
- Triplet-Encoded Prebiotic RNA Aminoacylation.Journal of the American Chemical Society · 2023Article
- Experimental Tests of the Virtual Circular Genome Model for Nonenzymatic RNA Replication.Journal of the American Chemical Society · 2023Article
- The Origin of Translation: Bridging the Nucleotides and Peptides.International journal of molecular sciences · 2022Review
- Hybridization kinetics of out-of-equilibrium mixtures of short RNA oligonucleotides.Nucleic acids research · 2022Article
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6 authors.
Funding
Abstract
The first ribozymes are thought to have emerged at a time when RNA replication proceeded via nonenzymatic template copying processes. However, functional RNAs have stable folded structures, and such structures are much more difficult to copy than short unstructured RNAs. How can these conflicting requirements be reconciled? Also, how can the inhibition of ribozyme function by complementary template strands be avoided or minimized? Here, we show that short RNA duplexes with single-stranded overhangs can be converted into RNA stem loops by nonenzymatic cross-strand ligation. We then show that loop-closing ligation reactions enable the assembly of full-length functional ribozymes without any external template. Thus, one can envisage a potential pathway whereby structurally complex functional RNAs could have formed at an early stage of evolution when protocell genomes might have consisted only of collections of short replicating oligonucleotides.
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