Evidence map›Paper›PMID 35880022›Full record

ArticleCell reports methods2022

An automated do-it-yourself system for dynamic stem cell and organoid culture in standard multi-well plates.

Julia Tischler, Zoe Swank, Hao-An Hsiung, Stefano Vianello, Matthias P Lutolf, Sebastian J Maerkl

Erratum issuedOpen access · goldAbstract read
In one paragraph

Article in Cell reports methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.0field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Incubator-Free Organoid Culture in a Sealed Recirculatory System.bioRxiv : the preprint server for biology · 2025
    Article
  5. Article
  6. Article
  7. Nanoplasmonic Single-Tumoroid Microarray for Real-Time Secretion Analysis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024
    Article
  8. Review
  9. Article
  10. Article
  11. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Julia TischlerLaboratory of Biological Network Characterization, Institute of Bioengineering, School of Engineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, 1015 Vaud, Switzerland.
Zoe SwankLaboratory of Biological Network Characterization, Institute of Bioengineering, School of Engineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, 1015 Vaud, Switzerland.
Hao-An HsiungLaboratory of Stem Cell Bioengineering, Institute of Bioengineering, School of Life Sciences and School of Engineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, 1015 Vaud, Switzerland.
Stefano VianelloLaboratory of Stem Cell Bioengineering, Institute of Bioengineering, School of Life Sciences and School of Engineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, 1015 Vaud, Switzerland.
Matthias P LutolfLaboratory of Stem Cell Bioengineering, Institute of Bioengineering, School of Life Sciences and School of Engineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, 1015 Vaud, Switzerland.
Sebastian J MaerklLaboratory of Biological Network Characterization, Institute of Bioengineering, School of Engineering, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, 1015 Vaud, Switzerland.
École Polytechnique Fédérale de Lausanne · CHBrigham and Women's Hospital · USRoche (Switzerland) · CH

Funding

A Pragmatic Trial of Lumbar Image Reporting with Epidemiology (LIRE)UH2AT007766 · NCCIH · UNIVERSITY OF WASHINGTON · PI JARVIK, JEFFREY G · 2012 to 2012
$838k
NCCIH NIH HHS UH2 AT007766
6 · The paper itself

Abstract

We present a low-cost, do-it-yourself system for complex mammalian cell culture under dynamically changing medium formulations by integrating conventional multi-well tissue culture plates with simple microfluidic control and system automation. We demonstrate the generation of complex concentration profiles, enabling the investigation of sophisticated input-response relations. We further apply our automated cell-culturing platform to the dynamic stimulation of two widely employed stem-cell-based

Indexed as

Germ LayersStem CellsAnimalsCell DifferentiationCells, CulturedMammalsMiceOrganoidscomplex cell culture in standard multi-well platesfully automated media exchangesfully automated system for complex mammalian cell culturemammalian cell culture under dynamically changing medium compositionsmedium formulation in real timemicrofluidic controlmouse 3D gastruloid culturemouse embryonic stem cell cultureorganoid cultureprogrammable cell culture system

Identifiers

PMID35880022
PMCPMC9308133
OpenAlexW4283774558

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.