ArticleMicrobial cell factories2022
Deletion of NTH1 and HSP12 increases the freeze-thaw resistance of baker's yeast in bread dough.
Article in Microbial cell factories, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed, 11 citations in OpenAlex.
- Yeast mitochondrial adaptation to freeze thaw stress depends on pre freeze fermentation state.NPJ science of food · 2026Article
- Enhancing freeze-thaw tolerance in baker's yeast: strategies and perspectives.Food science and biotechnology · 2024Review
- Diverse signatures of convergent evolution in cactus-associated yeasts.PLoS biology · 2024Article
- Article
- Diverse signatures of convergent evolution in cacti-associated yeasts.bioRxiv : the preprint server for biology · 2023Article
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2 authors at 1 institution in 1 country.
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Abstract
backgroundThe intracellular molecule trehalose in Saccharomyces cerevisiae may have a major protective function under extreme environmental conditions. NTH1 is one gene which expresses trehalase to degrade trehalose. Small heat shock protein 12 (HSP12 expressed) plays a role in protecting membranes and enhancing freezing stress tolerance.
resultsAn optimized S. cerevisiae CRISPR-Cpf1 genome-editing system was constructed. Multiplex genome editing using a single crRNA array was shown to be functional. NTH1 or/and HSP12 knockout in S. cerevisiae enhanced the freezing stress tolerance and improved the leavening ability after freezing and thawing.
conclusionsDeleting NTH1 in the combination with deleting HSP12 would strengthen the freezing tolerance and protect the cell viability from high rates of death in longer-term freezing. It provides valuable insights for breeding novel S. cerevisiae strains for the baking industry through a more precise, speedy, and economic genome-editing system.
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