ArticleVeterinary sciences2022
External Ear Canal Evaluation in Dogs with Chronic Suppurative Otitis Externa: Comparison of Direct Cytology, Bacterial Culture and 16S Amplicon Profiling.
Article in Veterinary sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 18 citations in OpenAlex.
- Evaluation of Dual-Near-Infrared-Wavelength Photobiomodulation Therapy for the Treatment of Canine Otitis Externa: A Prospective Multicenter Pilot Study.Animals : an open access journal from MDPI · 2026Article
- Probiotic Engraftment and Suppression of Canine Otitis Externa Pathogens by Probiotic Ear Drops.Veterinary dermatology · 2026Article
- Culture and Metagenomic Insights into the Ear Microbiota in Dogs with Healthy Ears and Otitis Externa.Veterinary sciences · 2026Article
- Bacterial Agents and Antimicrobial-Resistance Patterns in Canine Otitis Externa.Animals : an open access journal from MDPI · 2025Article
- Bacterial culture and antimicrobial susceptibility testing - basic information that every veterinary practitioner should know.The Canadian veterinary journal = La revue veterinaire canadienne · 2025Article
- Brachycephaly, Ear Anatomy, and Co-Does Size Matter? A Retrospective Study on the Influence of Size-Dependent Features Regarding Canine Otitis Externa.Animals : an open access journal from MDPI · 2025Article
- Antimicrobial Resistance and Risk Factors of Canine Bacterial Skin Infections.Pathogens (Basel, Switzerland) · 2025Article
- Fungal dysbiosis following antibacterial monotherapy in canine otitis externa.The Journal of small animal practice · 2025Article
- Molecular Epidemiological Characteristics ofAntibiotics (Basel, Switzerland) · 2025Article
- Future Challenge: Assessing the Antibiotic Susceptibility Patterns ofAntibiotics (Basel, Switzerland) · 2024Article
- Antibacterial effects of chitosan-based hydrogels containing Trachyspermum ammi essential oil on pathogens isolated from dogs with otitis externa.BMC veterinary research · 2024Article
- Microbiological Survey and Evaluation of Antimicrobial Susceptibility Patterns of Microorganisms Obtained from Suspect Cases of Canine Otitis Externa in Gran Canaria, Spain.Animals : an open access journal from MDPI · 2024Article
- The role of the microbiome in allergic dermatitis-related otitis externa: a multi-species comparative review.Frontiers in veterinary science · 2024Review
- Review
- Antimicrobial Activity ofVeterinary sciences · 2023Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A discrepancy between cytology and bacterial culture methods is sometimes observed in canine otitis externa. The objective of this study was to compare results from cytology, bacterial culture and 16S amplicon profiling. Twenty samples from 16 dogs with chronic suppurative otitis externa were collected. A direct cytological evaluation was carried out during the consultations. Aerobic bacterial culture and susceptibility were performed by an external laboratory used in routine practice. For 16S amplicon profiling, DNA was extracted and the hypervariable segment V1−V3 of the 16S rDNA was amplified and then sequenced with a MiSeq Illumina sequence carried out by the Mothur software using the SILVA database. A good correlation between cytology and bacterial culture was observed in 60% of the samples. Some bacterial species revealed by bacterial culture were present with low relative abundance (<10%) in 16S amplicon profiling. Some bacterial species revealed by the 16S amplicon profiling analysis were not identified with culture; most of the time, the offending species was a Corynebacterium. To conclude, a careful interpretation of the results of bacterial culture should be made and always be in agreement with the cytology. The 16S amplicon profiling method appears to be a more sensitive method for detecting strains present in suppurative otitis but does not provide information on bacterial susceptibility.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.