ArticleVeterinary sciences2022
Validation of a Commercial Indirect ELISA Kit for the Detection of
Article in Veterinary sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Development of a recombinant glycoprotein E-based ELISA for detection of antibodies against Bovine alphaherpesvirus 1: a DIVA compliant.Veterinary research communications · 2026Article
- A newly developed rapid K39-based LeishmaCHECK Ab ELISA for high-throughput serological screening of canine leishmaniasis.Current research in parasitology & vector-borne diseases · 2026Article
- Robust BICP0-gB indirect ELISA for the accurate diagnosis of bovine alphaherpesvirus 1 infections.Veterinary and animal science · 2025Article
- Development of a New Indirect ELISA Test for the Detection of Anti-Feline Coronavirus Antibodies in Cats.Veterinary sciences · 2025Article
- A New Rapid Indirect ELISA Test for Serological Diagnosis of Feline Immunodeficiency.Veterinary sciences · 2025Article
- OASIS evaluation of the French laboratory diagnostic surveillance system: right people, right techniques but imperfect use.Frontiers in veterinary science · 2025Article
- Evaluation of a newly developed rapid ELISA to detect anti-Ehrlichia canis antibodies in dogs.Parasite (Paris, France) · 2025Article
- Feline Leukemia Virus in Cats: A Novel Rapid ELISA Assay for p27 Antigen Detection.Veterinary medicine international · 2025Article
- Infectious bovine rhinotracheitis: Unveiling the hidden threat to livestock productivity and global trade.Open veterinary journal · 2024Review
- Serodiagnosis of nasal myasis in camels (Camelus dromedaries) in Egypt using third larval instar affinity-purified glycoprotein.Veterinary research communications · 2024Article
- Article
- Simultaneous detection of bovine viral diarrhea virus (BVDV) and bovine herpesvirus 1 (BoHV-1) using recombinase polymerase amplification.Scientific reports · 2024Article
- Genomic Characterization of a Wild-TypeViruses · 2024Article
- Humoral Immune Response of Mice against a Vaccine Candidate Composed of a Chimera of gB of Bovine Alphaherpesviruses 1 and 5.Vaccines · 2023Article
- Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
In this study, we validated a commercial indirect enzyme-linked immunosorbent assay (ELISA) to detect antibodies to glycoprotein E (gE) of Bovine alphaherpesvirus 1 (BoHV-1) in bulk milk (BM) samples using the OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. The assay performance characteristics were evaluated using a panel of positive (n = 36) and negative (n = 80) samples with known infectious bovine rhinotracheitis (IBR) status. The assay showed adequate repeatability (within-run and between-run), with a coefficient of variability (CV%) of replicates below 30%; only two 1:40 diluted samples had a CV% above 20%. Additionally, an agreement analysis of the qualitative results of replicates led to a Gwet’s agreement coefficient of 0.99 (95% confidence interval (CI): 0.96−1.00, p < 0.001). The estimated diagnostic sensitivity (DSe) and diagnostic specificity (DSp) were 100% (95% CI: 90.3−100%) and 97.5% (95% CI: 91.3−99.7%), respectively. Overall, a good level of agreement was observed between the assay results and the true IBR status of samples (weighted Cohen’s κ: 0.96, 95% CI: 0.78−1.00). The findings demonstrate that the indirect ELISA kit validated here is an easy-to-use and economical method to differentiate infected and gE-deleted marker vaccine-immunised animals using BM samples.
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Registered trials
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