ArticleGels (Basel, Switzerland)2022
Gellan Gum Is a Suitable Biomaterial for Manual and Bioprinted Setup of Long-Term Stable, Functional 3D-Adipose Tissue Models.
Article in Gels (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Engineering 3D adipose cell models by support bath bioprinting of mature adipocytes in gellan gum hydrogels.Engineering in life sciences · 2026Article
- Organ-Specific Strategies in Bioprinting: Addressing Translational Challenges in the Heart, Liver, Kidney, and Pancreas.Journal of functional biomaterials · 2025Review
- Animal-Free Setup of a 3D Mature Adipocyte-Macrophage Co-Culture to Induce Inflammation In Vitro.Advanced healthcare materials · 2025Article
- Exploring Morphological and Molecular Properties of Different Adipose Cell Models: Monolayer, Spheroids, Gellan Gum-Based Hydrogels, and Explants.Macromolecular bioscience · 2025Article
- Dynamically cultured, differentiated bovine adipose-derived stem cell spheroids as building blocks for biofabricating cultured fat.Nature communications · 2024Article
- Robot-based 6D bioprinting for soft tissue biomedical applications.Engineering in life sciences · 2024Article
- Biofabrication's Contribution to the Evolution of Cultured Meat.Advanced healthcare materials · 2024Review
- Gums as Macromolecular Crowding Agents in Human Skin Fibroblast Cultures.Life (Basel, Switzerland) · 2024Article
- Flexible Allyl-Modified Gelatin Photoclick Resin Tailored for Volumetric Bioprinting of Matrices for Soft Tissue Engineering.Advanced healthcare materials · 2023Article
- Biopolymers for Tissue Engineering: Crosslinking, Printing Techniques, and Applications.Gels (Basel, Switzerland) · 2023Review
- Functional biomaterials for biomimetic 3D in vitro tumor microenvironment modeling.In vitro models · 2023Review
- How Mechanical and Physicochemical Material Characteristics Influence Adipose-Derived Stem Cell Fate.International journal of molecular sciences · 2023Review
- Bioprinting of 3D Adipose Tissue Models Using a GelMA-Bioink with Human Mature Adipocytes or Human Adipose-Derived Stem Cells.Gels (Basel, Switzerland) · 2022Article
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Authors and funding
5 authors.
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Abstract
Due to its wide-ranging endocrine functions, adipose tissue influences the whole body's metabolism. Engineering long-term stable and functional human adipose tissue is still challenging due to the limited availability of suitable biomaterials and adequate cell maturation. We used gellan gum (GG) to create manual and bioprinted adipose tissue models because of its similarities to the native extracellular matrix and its easily tunable properties. Gellan gum itself was neither toxic nor monocyte activating. The resulting hydrogels exhibited suitable viscoelastic properties for soft tissues and were stable for 98 days in vitro. Encapsulated human primary adipose-derived stem cells (ASCs) were adipogenically differentiated for 14 days and matured for an additional 84 days. Live-dead staining showed that encapsulated cells stayed viable until day 98, while intracellular lipid staining showed an increase over time and a differentiation rate of 76% between days 28 and 56. After 4 weeks of culture, adipocytes had a univacuolar morphology, expressed perilipin A, and secreted up to 73% more leptin. After bioprinting establishment, we demonstrated that the cells in printed hydrogels had high cell viability and exhibited an adipogenic phenotype and function. In summary, GG-based adipose tissue models show long-term stability and allow ASCs maturation into functional, univacuolar adipocytes.
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