ArticleeLife2022
Self-organization of kinetochore-fibers in human mitotic spindles.
Article in eLife, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 24 citations in OpenAlex.
- Structure and function of the outer kinetochore.Nature reviews. Molecular cell biology · 2026Review
- Towards a systems-level view of the microtubule cytoskeleton and its functions in physiology and disease.Nature reviews. Molecular cell biology · 2026Review
- Analyses of bent spindles reveal the mechanics of anaphase B in fission yeast.Molecular biology of the cell · 2026Article
- Human mitotic spindles as active liquid crystals: From collective behaviors to discrete filaments.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- The kinetochore corona orchestrates chromosome congression through transient microtubule interactions.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Eg5 activity and density-driven bundling organize the human metaphase mitotic spindle independently of spindle bipolarity.bioRxiv : the preprint server for biology · 2026Article
- Analyses of bent spindles reveal the mechanics of anaphase B in fission yeast.bioRxiv : the preprint server for biology · 2025Article
- <italic>Zea mays</italic> Meiotic Spindle Ultrastructure Reveals Kinetochore-Microtubule Interface and Embedded Membrane Components.Cytogenetic and genome research · 2025Article
- Microtubule choreography: spindle self-organization during cell division.Biophysical reviews · 2024Review
- Long-range repulsion between chromosomes in mammalian oocyte spindles.Science advances · 2024Article
- CFDP1 regulates the stability of pericentric heterochromatin thereby affecting RAN GTPase activity and mitotic spindle formation.PLoS biology · 2024Article
- Microtubule nucleation for spindle assembly: one molecule at a time.Trends in biochemical sciences · 2023Review
- Principles and dynamics of spindle assembly checkpoint signalling.Nature reviews. Molecular cell biology · 2023Review
- Kinetochore-fiber lengths are maintained locally but coordinated globally by poles in the mammalian spindle.eLife · 2023Article
- BUB-1 and CENP-C recruit PLK-1 to control chromosome alignment and segregation during meiosis I ineLife · 2023Article
- α-Tubulin detyrosination links the suppression of MCAK activity with taxol cytotoxicity.The Journal of cell biology · 2023Article
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- Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.Cell reports · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During eukaryotic cell division, chromosomes are linked to microtubules (MTs) in the spindle by a macromolecular complex called the kinetochore. The bound kinetochore microtubules (KMTs) are crucial to ensuring accurate chromosome segregation. Recent reconstructions by electron tomography (Kiewisz et al., 2022) captured the positions and configurations of every MT in human mitotic spindles, revealing that roughly half the KMTs in these spindles do not reach the pole. Here, we investigate the processes that give rise to this distribution of KMTs using a combination of analysis of large-scale electron tomography, photoconversion experiments, quantitative polarized light microscopy, and biophysical modeling. Our results indicate that in metaphase, KMTs grow away from the kinetochores along well-defined trajectories, with the speed of the KMT minus ends continually decreasing as the minus ends approach the pole, implying that longer KMTs grow more slowly than shorter KMTs. The locations of KMT minus ends, and the turnover and movements of tubulin in KMTs, are consistent with models in which KMTs predominately nucleate de novo at kinetochores in metaphase and are inconsistent with substantial numbers of non-KMTs being recruited to the kinetochore in metaphase. Taken together, this work leads to a mathematical model of the self-organization of kinetochore-fibers in human mitotic spindles.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.