Evidence map›Paper›PMID 35875822›Full record

ArticleJournal of chromatography open2022

Quantification of N, N' N"-triethylenethiophosphoramide, N, N"-triethylenephosphoramide, cyclophosphamide, and 4-hydroxy-cyclophosphamide in microvolume human plasma to support neonatal and pediatric drug studies.

Liusheng Huang, Beth Apsel Winger, Vincent Cheah, David Gingrich, Florence Marzan, Ying Lu, Jennifer C Cooper, Francesca Aweeka, Janel Long-Boyle

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of chromatography open, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Liusheng HuangDrug Research Unit, Department of Clinical Pharmacy, University of California San Francisco, USA.
Beth Apsel WingerDepartment of Pediatrics, University of California San Francisco.
Vincent CheahDrug Research Unit, Department of Clinical Pharmacy, University of California San Francisco, USA.
David GingrichDrug Research Unit, Department of Clinical Pharmacy, University of California San Francisco, USA.
Florence MarzanDrug Research Unit, Department of Clinical Pharmacy, University of California San Francisco, USA.
Ying LuDrug Research Unit, Department of Clinical Pharmacy, University of California San Francisco, USA.
Jennifer C CooperDepartment of Pediatrics, University of Colorado Anschutz Medical Campus.
Francesca AweekaDrug Research Unit, Department of Clinical Pharmacy, University of California San Francisco, USA.
Janel Long-BoyleDrug Research Unit, Department of Clinical Pharmacy, University of California San Francisco, USA.
University of California, San Francisco · USUniversity of Colorado Anschutz Medical Campus · US

Funding

Translational InformaticsP30CA082103 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Jennifer Leigh Clarke · 1999 to 2026
$209.7M
Virology CoreP30AI027763 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI STEVEN Grant DEEKS · 1988 to 2026
$93.6M
Clinical and Transtional Science InstituteKL2TR000143 · NCATS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI GRANDIS, JENNIFER RUBIN · 2012 to 2015
$11.9M
NCATS NIH HHS KL2 TR000143NCI NIH HHS P30 CA082103NIAID NIH HHS P30 AI027763
6 · The paper itself

Abstract

N, N' N"-triethylenethiophosphoramide (thiotepa) and cyclophosphamide (CP) are alkylating agents used for a variety of malignant and non-malignant disorders. Both drugs are metabolized by cytochrome P450 enzymes to form active metabolites. To support pharmacokinetic studies of thiotepa and CP in children, we sought to develop assays to determine parent drug and metabolite concentration in small volume plasma samples. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used for assay development. CP metabolite 4-hydroxycyclophosphamide (4OHCP) was converted to the more stable semicarbazone derivative (4OHCP-SCZ) for quantitation. Samples (10 μL) were extracted by solid-phase extraction and injected onto the LC-MS/MS system equipped with a pentafluorophenyl reverse phase column (2.1 × 50 mm, 2.7 μm). Electrospray ionization in positive mode was used for detection. Multiple reaction monitoring of the precursor-to-product ion transitions

Indexed as

4-hydroxycyclophosphamidecyclophosphamidepediatricplasmatepaThiotepa

Identifiers

PMID35875822
PMCPMC9302610
OpenAlexW4281741395

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.