Evidence map›Paper›PMID 35874920›Full record

ArticleiScience2022

Mechanistic elucidation of freezing-induced surface decomposition of aluminum oxyhydroxide adjuvant.

Jiahuan Li, Ge Yu, Zhihui Liang, Min Li, Chen Chen, Xin Li, Yiyang Guo, Cheng Yang, Yang Liu, Caiqiao Zhang and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 7 citations in OpenAlex.

  1. Macrophage-MediatedACS applied materials & interfaces · 2026
    Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 1 institution in 1 country.

Jiahuan LiState Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, 116024 Dalian, China.
Ge YuState Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, 116024 Dalian, China.
Zhihui LiangState Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, 116024 Dalian, China.
Min LiState Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, 116024 Dalian, China.
Chen ChenSchool of Bioengineering, Dalian University of Technology, 2 Linggong Road, 116024 Dalian, China.
Xin LiSchool of Chemical Engineering, Dalian University of Technology, 2 Linggong Road, 116024 Dalian, China.
Yiyang GuoSchool of Chemical Engineering, Dalian University of Technology, 2 Linggong Road, 116024 Dalian, China.
Cheng YangSchool of Chemistry, Dalian University of Technology, 2 Linggong Road, 116024 Dalian, China.
Yang LiuState Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, 116024 Dalian, China.
Caiqiao ZhangNCPC Genetech Biotechnology Co., Ltd., Shijiazhuang 050035, P. R. China.
Weiting ZhangNCPC Genetech Biotechnology Co., Ltd., Shijiazhuang 050035, P. R. China.
Jiaxu LiuSchool of Chemical Engineering, Dalian University of Technology, 2 Linggong Road, 116024 Dalian, China.
Xuehu MaSchool of Chemical Engineering, Dalian University of Technology, 2 Linggong Road, 116024 Dalian, China.
Changying XueSchool of Bioengineering, Dalian University of Technology, 2 Linggong Road, 116024 Dalian, China.
Bingbing SunState Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, 116024 Dalian, China.
Dalian University of Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The freezing-induced aggregation of aluminum-based (Alum) adjuvants has been considered as the most important cause of reduced vaccine potency. However, the intrinsic properties that determine the functionality of Alum after freezing have not been elucidated. In this study, we used engineered aluminum oxyhydroxide nanoparticles (AlOOH NPs) and demonstrated that cryogenic freezing led to the mechanical pressure-mediated reduction of surface hydroxyl. The sugar-based surfactant, octyl glucoside (OG), was demonstrated to shield AlOOH NPs from the freezing-induced loss of hydroxyl content and the aggregation through the reduction of recrystallization-induced mechanical stress. As a result, the antigenic adsorption property of frozen AlOOH NPs could be effectively protected. When hepatitis B surface antigen (HBsAg) was adjuvanted with OG-protected frozen AlOOH NPs in mice, the loss of immunogenicity was inhibited. These findings provide insights into the freezing-induced surface decomposition of Alum and can be translated to design of protectants to improve the stability of vaccines.

Indexed as

BiotechnologyInorganic materialsMaterials chemistryMaterials science

Identifiers

PMID35874920
PMCPMC9301878
OpenAlexW4281390202

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.