Evidence map›Paper›PMID 35874686›Full record

ArticleFrontiers in immunology2022

The Culture Dish Surface Influences the Phenotype and Dissociation Strategy in Distinct Mouse Macrophage Populations.

Qiaoling Song, Yazhuo Zhang, Mingming Zhou, Yuting Xu, Qianyue Zhang, Lihong Wu, Shan Liu, Minghui Zhang, Lei Zhang, Zhihua Wu and 3 more

Open access · goldAbstract read
In one paragraph

Article in Frontiers in immunology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
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  4. Inhibition of the HFrontiers in immunology · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 3 countries.

Qiaoling SongSchool of Medicine and Pharmacy, Ocean University of China, Qingdao, China.
Yazhuo ZhangSchool of Medicine and Pharmacy, Ocean University of China, Qingdao, China.
Mingming ZhouInnovation Platform of Marine Drug Screening and Evaluation, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
Yuting XuSchool of Medicine and Pharmacy, Ocean University of China, Qingdao, China.
Qianyue ZhangSchool of Medicine and Pharmacy, Ocean University of China, Qingdao, China.
Lihong WuSchool of Medicine and Pharmacy, Ocean University of China, Qingdao, China.
Shan LiuInnovation Platform of Marine Drug Screening and Evaluation, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
Minghui ZhangSchool of Medicine and Pharmacy, Ocean University of China, Qingdao, China.
Lei ZhangSchool of Medicine and Pharmacy, Ocean University of China, Qingdao, China.
Zhihua WuCollege of Food Science and Engineering, Ocean University of China, Qingdao, China.
Weixun PengFaculty of Medicine, Imperial College London, London, United Kingdom.
Xutao LiuSamueli School of Engineering, University of California Los Angeles, Los Angeles, CA, United States.
Chenyang ZhaoSchool of Medicine and Pharmacy, Ocean University of China, Qingdao, China.
Ocean University of China · CNQingdao National Laboratory for Marine Science and Technology · CNImperial College London · GBUniversity of California, Los Angeles · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The nature of the culture dish surface and the technique used to detach adherent cells could very likely influence the cell viability and cell membrane protein integrity of harvested macrophages. Several previous studies assessed the detachment efficacies of enzymatic and non-enzymatic methods for harvesting the single cell suspensions of macrophages, but a comprehensive study assessing different dissociation methods and culture conditions for detaching functionally different macrophage populations has not yet been reported. In this study,

Indexed as

Cell Culture TechniquesMacrophagesAnimalsEdetic AcidGranulocyte-Macrophage Colony-Stimulating FactorLipopolysaccharidesMacrophage Colony-Stimulating FactorMicePhenotypeTrypsinEdetic AcidGranulocyte-Macrophage Colony-Stimulating FactorLipopolysaccharidesMacrophage Colony-Stimulating FactorTrypsinculture dish surfacedissociation methodsLPS challengemacrophage polarizationtranscriptome sequencing

Identifiers

PMID35874686
PMCPMC9299442
OpenAlexW4286631758

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.