Evidence map›Paper›PMID 35873535›Full record

ArticleStem cells international2022

Umbilical Cord Mesenchymal Stem Cell-Derived Small Extracellular Vesicles Deliver miR-21 to Promote Corneal Epithelial Wound Healing through PTEN/PI3K/Akt Pathway.

Xiaolong Liu, Xuran Li, Guangyuan Wu, Pengfei Qi, Yanyan Zhang, Zhiyu Liu, Xinyue Li, Yu Yu, Xiangmei Ye, Yang Li and 12 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells international, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
46citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

46 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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  6. Article
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  9. Diabetic keratopathy and nuclear proteins (Review).Molecular medicine reports · 2026
    Review
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  15. Article
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  18. Biomedical Application of MSCs in Corneal Regeneration and Repair.International journal of molecular sciences · 2025
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors.

Xiaolong LiuCentral Laboratory, First Affiliated Hospital, Harbin Medical University, Harbin, China.ORCID https://orcid.org/0000-0002-1359-4376
Xuran LiEye Hospital, First Affiliated Hospital, Harbin Medical University, Harbin, China.ORCID https://orcid.org/0000-0001-6008-9316
Guangyuan WuCentral Laboratory, First Affiliated Hospital, Harbin Medical University, Harbin, China.ORCID https://orcid.org/0000-0003-1334-9634
Pengfei QiCentral Laboratory, First Affiliated Hospital, Harbin Medical University, Harbin, China.ORCID https://orcid.org/0000-0001-7329-9419
Yanyan ZhangEye Hospital, First Affiliated Hospital, Harbin Medical University, Harbin, China.
Zhiyu LiuDepartment of Laboratory Diagnostics, First Affiliated Hospital, Harbin Medical University, Harbin, China.
Xinyue LiEye Hospital, First Affiliated Hospital, Harbin Medical University, Harbin, China.
Yu YuEye Hospital, First Affiliated Hospital, Harbin Medical University, Harbin, China.
Xiangmei YeCentral Laboratory, First Affiliated Hospital, Harbin Medical University, Harbin, China.
Yang LiCentral Laboratory, First Affiliated Hospital, Harbin Medical University, Harbin, China.
Dongguang YangCentral Laboratory, First Affiliated Hospital, Harbin Medical University, Harbin, China.
Yueqiu TengCentral Laboratory, First Affiliated Hospital, Harbin Medical University, Harbin, China.
Ce ShiCentral Laboratory, First Affiliated Hospital, Harbin Medical University, Harbin, China.
Xin JinEye Hospital, First Affiliated Hospital, Harbin Medical University, Harbin, China.
Sen QiDepartment of Hematology, First Affiliated Hospital, Harbin Medical University, Harbin, China.
Yuting LiuEye Hospital, First Affiliated Hospital, Harbin Medical University, Harbin, China.
Shudan WangEye Hospital, First Affiliated Hospital, Harbin Medical University, Harbin, China.
Ying LiuEye Hospital, First Affiliated Hospital, Harbin Medical University, Harbin, China.
Fenglin CaoCentral Laboratory, First Affiliated Hospital, Harbin Medical University, Harbin, China.
Qingran KongCollege of Life Science, Northeast Agricultural University, Harbin, China.
Zhenkun WangCentral Laboratory, First Affiliated Hospital, Harbin Medical University, Harbin, China.ORCID https://orcid.org/0000-0003-4894-0029
Hong ZhangEye Hospital, First Affiliated Hospital, Harbin Medical University, Harbin, China.ORCID https://orcid.org/0000-0003-4179-5666

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objective: Rapid restoration of corneal epithelium integrity after injury is particularly important for preserving corneal transparency and vision. Mesenchymal stem cells (MSCs) can be taken into account as the promising regenerative therapeutics for improvement of wound healing processes based on the variety of the effective components. The extracellular vesicles form MSCs, especially exosomes, have been considered as important paracrine mediators though transferring microRNAs into recipient cell. This study investigated the mechanism of human umbilical cord MSC-derived small extracellular vesicles (HUMSC-sEVs) on corneal epithelial wound healing. Methods: HUMSC-sEVs were identified by transmission electron microscopy, nanoparticle tracking analysis, and Western blot. Corneal fluorescein staining and histological staining were evaluated in a corneal mechanical wound model. Changes in HCEC proliferation after HUMSC-sEVs or miR-21 mimic treatment were evaluated by CCK-8 and EdU assays, while migration was assessed by in vitro scratch wound assay. Full-length transcriptome sequencing was performed to identify the differentially expressed genes associated with HUMSC-sEVs treatment, followed by validation via real-time PCR and Western blot. Results: The sEVs derived from HUMSCs can significantly promote corneal epithelial cell proliferation, migration in vitro, and corneal epithelial wound healing in vivo. Similar effects were obtained after miR-21 transfection, while the beneficial effects of HUMSC-sEVs were partially negated by miR-21 knockdown. Results also show that the benefits are associated with decreased PTEN level and activated the PI3K/Akt signaling pathway in HCECs. Conclusion: HUMSC-sEVs could enhance the recovery of corneal epithelial wounds though restraining PTEN by transferring miR-21 and may represent a promising novel therapeutic agent for corneal wound repair.

Identifiers

PMID35873535
PMCPMC9303509

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.