ArticleNanophotonics (Berlin, Germany)2022
Monolithic dual-wedge prism-based spectroscopic single-molecule localization microscopy.
Article in Nanophotonics (Berlin, Germany), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 15 citations in OpenAlex.
- Spectroscopic DNA-PAINT for simultaneous multiplexed super-resolution microscopy.bioRxiv : the preprint server for biology · 2026Article
- Maximizing photon utilization in spectroscopic single-molecule localization microscopy using symmetrically dispersed dual-wedge prisms.Npj imaging · 2026Article
- Spectroscopic Super-resolution Imaging of DNA Molecules using Intrinsic Contrast.Journal of visualized experiments : JoVE · 2026Article
- Orthogonally Dispersed Spectroscopic Single-Molecule Localization Microscopy.Nanophotonics (Berlin, Germany) · 2026Article
- Deep learning-based spectroscopic single-molecule localization microscopy.Journal of biomedical optics · 2024Article
- Physically informed Monte Carlo simulation of dual-wedge prism-based spectroscopic single-molecule localization microscopy.Journal of biomedical optics · 2024Article
- Spectral precision improvement with demagnifying spectral images in spectroscopic nanoscopy.Journal of the Optical Society of America. A, Optics, image science, and vision · 2024Article
- Switchable and Functional Fluorophores for Multidimensional Single-Molecule Localization Microscopy.Chemical & biomedical imaging · 2023Review
- Spectroscopic single-molecule localization microscopy: applications and prospective.Nano convergence · 2023Review
- Enabling Spectrally Resolved Single-Molecule Localization Microscopy at High Emitter Densities.Nano letters · 2022Article
- Deep learning in single-molecule imaging and analysis: recent advances and prospects.Chemical science · 2022Review
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
By manipulating the spectral dispersion of detected photons, spectroscopic single-molecule localization microscopy (sSMLM) permits concurrent high-throughput single-molecular spectroscopic analysis and imaging. Despite its promising potential, using discrete optical components and managing the delicate balance between spectral dispersion and spatial localization compromise its performance, including non-uniform spectral dispersion, high transmission loss of grating, high optical alignment demands, and reduced precision. We designed a dual-wedge prism (DWP)-based monolithic imaging spectrometer to overcome these challenges. We optimized the DWP for spectrally dispersing focused beam without deviation and with minimal wavefront error. We integrated all components into a compact assembly, minimizing total transmission loss and significantly reducing optical alignment requirements. We show the feasibility of DWP using ray-tracing and numerical simulations. We validated our numerical simulations by experimentally imaging individual nanospheres and confirmed that DWP-sSMLM achieved much improved spatial and spectral precisions of grating-based sSMLM. We also demonstrated DWP-sSMLM in 3D multi-color imaging of cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.