ArticleArchives of gynecology and obstetrics2022
In vitro growth (IVG) of human ovarian follicles in frozen thawed ovarian cortex tissue culture supplemented with follicular fluid under hypoxic conditions.
Article in Archives of gynecology and obstetrics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed, 13 citations in OpenAlex.
- Optimal autofluorescence blocking for reliable immunofluorescence in human ovarian samples: A comparison of 4 blocking kits.Biochemistry and biophysics reports · 2026Article
- Transcriptomic insights into ovine ovarian response to extended in vitro culture and cryopreservation.Journal of assisted reproduction and genetics · 2026Article
- Geographic patterns of ovarian tissue cryopreservation utilisation across age groups in a nationwide multicentre cohort.Human reproduction open · 2026Article
- Expression of growth factors in buffalo ovarian tissue across different follicular developmental stages.Archives of gynecology and obstetrics · 2025Article
- Ovarian tissue cryopreservation for fertility preservation: a two-decade single-center experience with 451 children and adolescents.Reproductive biology and endocrinology : RB&E · 2025Article
- Study of Ovarian Damage in Piglets in an Experimental Model of Neonatal Asphyxia.Children (Basel, Switzerland) · 2025Article
- High FSH levels impair VEGF secretion of human, frozen-thawed ovarian cortical tissue in vitro.Scientific reports · 2024Article
- Influence of ovarian stromal cells on human ovarian follicle growth in a 3D environment.Human reproduction open · 2024Article
- Comparison of angiogenic potential in vitrified vs. slow frozen human ovarian tissue.Scientific reports · 2023Article
- The effect of high-throughput vitrification of human ovarian cortex tissue on follicular viability: a promising alternative to conventional slow freezing?Archives of gynecology and obstetrics · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDespite its clinical success rates, transplantation after ovarian tissue cryopreservation (OTC) remains a matter of concern. Certain cancer subtypes may lead to the transfer of malignant cells when transplantation of affected ovarian tissue is conducted. IVG and subsequent isolation of vital follicles obtained from frozen thawed ovarian tissue for further in vitro maturation (IVM) would expand current fertility protection techniques while reducing the risk of retransplanting malignant cells.
methodsA total of 216 cortical biopsies from 3 patients were included in this study in 4 treatment groups. After freezing, thawing and 8 days of hypoxic tissue culture supplemented with different concentrations of human follicular fluid (HuFF) and follicle-stimulating hormone (FSH), follicles were isolated enzymatically and stained with calcein to determine follicular viability. Numbers and size of vital follicles were assessed by fluorescence microscopy (Ti2, Nikon) and specified by computer assisted, semi-automated measurement (NIS software, Nikon). To estimate the effect of in vitro culture on apoptosis, tissue sections were stained for nicked DNA (TUNEL) prior and after tissue culture.
resultsAnalysing 3025 vital follicles, we observed significant differences [P < 0.01] regarding follicle size when hypoxic tissue culture was supplemented with HuFF compared with the control group on day 1, individual follicles reached sizes > 100 µm.
conclusionsThe results implicate that HuFF contains valuable factors contributing to significant IVG of follicles in human ovarian tissue and could be regarded as an additional tool in personalized fertility restoration prior to retransplantation of ovarian tissue.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.