ArticlePlant biotechnology journal2022
Genetic modification of flavone biosynthesis in rice enhances biofilm formation of soil diazotrophic bacteria and biological nitrogen fixation.
Article in Plant biotechnology journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers, 1 of them a synthesis that pooled it.
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Who cites it
56 citing papers in PubMed, 1 synthesis or guideline pooled it, 111 citations in OpenAlex.
- Root colonization by beneficial rhizobacteria.FEMS microbiology reviews · 2024Pooled it
- Influence of sound vibrations on plant holobionts: physiological pathways linking root function and rhizospheric microbial interactions.Plant signaling & behavior · 2026Review
- Sphingobium yanoikuyae 41R9 Enhances Nitrogen Uptake by Modulating Transporter Genes and Root Development in Rapeseed.Plant, cell & environment · 2026Article
- Drought Stress Mediated Changes in Food Crops: Mechanisms and Remediation Strategies.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Plant-Mediated Production of Antibacterial Nanomaterials Fulfilling a Reactive Oxygen Species Strategy.International journal of molecular sciences · 2026Review
- Rational reduction of a sorghum SynCom that preserves growth promotion reveals flavonoid-mediated plant-microbe interactions.The New phytologist · 2026Article
- Harnessing plant-exuded prebiotics as a next-generation strategy for sustainable agriculture.Journal of integrative plant biology · 2026Review
- Engineered diazotrophs with host-inducible nitrogen supply systems: Transforming rice farming through innovative nitrogen biofertilizers.Journal of integrative plant biology · 2026Review
- Bag-controlled low-nitrogen fertilization induces root arbutin secretion to enrich rhizosphere Pseudomonas and enhance nitrogen fixation in peach.Plant communications · 2026Article
- A bacterial ally for nitrogen-fixing biofilm: enhancing the rhizosphere colonization ofApplied and environmental microbiology · 2026Article
- Advances in CRISPR Plant Applications.International journal of molecular sciences · 2026Review
- Plant-Associated Microbiomes: Crosstalk and Engineering to Improve Nutrient Use Efficiency (NUE) in Crops of Global Importance.Plants (Basel, Switzerland) · 2026Review
- Kaempferol drives genotype-specific microbiota Bacillaceae to enhance nitrogen acquisition in rapeseed.Journal of advanced research · 2026Article
- The plant microbiota: From theoretical advances to applications.Fundamental research · 2026Review
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Enhancing biological nitrogen fixation in rice paddy ecosystems: challenges, opportunities, and sustainable strategies.Frontiers in microbiology · 2026Review
- Stable foliar colonization of nanocoated nitrogen-fixing bacteria enhances crop nitrogen supply.Nature food · 2026Article
- Synthetic bacterium-facilitated colonization of nitrogen-fixing bacteria for remodeling the rhizosphere microbiome and improving plant yield.Microbiome · 2025Article
- The Potential of NGTs to Overcome Constraints in Plant Breeding and Their Regulatory Implications.International journal of molecular sciences · 2025Review
- Elucidating the growth-promoting factors of Bacillus amyloliquefaciens on rapeseed through metabolomics.BMC plant biology · 2025Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Improving biological nitrogen fixation (BNF) in cereal crops is a long-sought objective; however, no successful modification of cereal crops showing increased BNF has been reported. Here, we described a novel approach in which rice plants were modified to increase the production of compounds that stimulated biofilm formation in soil diazotrophic bacteria, promoted bacterial colonization of plant tissues and improved BNF with increased grain yield at limiting soil nitrogen contents. We first used a chemical screening to identify plant-produced compounds that induced biofilm formation in nitrogen-fixing bacteria and demonstrated that apigenin and other flavones induced BNF. We then used CRISPR-based gene editing targeting apigenin breakdown in rice, increasing plant apigenin contents and apigenin root exudation. When grown at limiting soil nitrogen conditions, modified rice plants displayed increased grain yield. Biofilm production also modified the root microbiome structure, favouring the enrichment of diazotrophic bacteria recruitment. Our results support the manipulation of the flavone biosynthetic pathway as a feasible strategy for the induction of biological nitrogen fixation in cereals and a reduction in the use of inorganic nitrogen fertilizers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.