ArticleNature communications2022
Construction of the axolotl cell landscape using combinatorial hybridization sequencing at single-cell resolution.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Evolution of an amphibian-specific family of epidermal differentiation-associated genes.Molecular biology and evolution · 2026Article
- A mammalian-like piRNA pathway in Axolotl reveals the origins of piRNA-directed DNA methylation.The EMBO journal · 2026Article
- A spatial transcriptomics comparison of the adult versus metamorphosed axolotl brain.Scientific data · 2026Article
- Retrotransposon Expression Is Upregulated in Adulthood and Suppressed during Regeneration of the Limb in the Axolotl (Ambystoma mexicanum).Advanced biology · 2025Article
- Environmental stress promotes the persistence of juvenile traits in olfactory neurons as a protective mechanism.iScience · 2025Article
- Applications and techniques of single-cell RNA sequencing across diverse species.Briefings in bioinformatics · 2025Review
- Advances and applications in single-cell and spatial genomics.Science China. Life sciences · 2025Review
- Suppressors of cGAS-STING are downregulated during fin-limb regeneration and aging in aquatic vertebrates.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2024Article
- A Multimodal Omics Framework to Empower Target Discovery for Cardiovascular Regeneration.Cardiovascular drugs and therapy · 2024Review
- Fast and flexible profiling of chromatin accessibility and total RNA expression in single nuclei using Microwell-seq3.Cell discovery · 2024Article
- Mapping Cell Atlases at the Single-Cell Level.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Evaluation of genetic demultiplexing of single-cell sequencing data from model species.Life science alliance · 2023Article
- Construction of a cross-species cell landscape at single-cell level.Nucleic acids research · 2023Article
- Characterization of immunoglobulinFrontiers in immunology · 2023Article
- Editorial: Machine learning-based methods for RNA data analysis-Volume II.Frontiers in genetics · 2022Article
Corrections and comments
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Authors and funding
26 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Mexican axolotl (Ambystoma mexicanum) is a well-established tetrapod model for regeneration and developmental studies. Remarkably, neotenic axolotls may undergo metamorphosis, a process that triggers many dramatic changes in diverse organs, accompanied by gradually decline of their regeneration capacity and lifespan. However, the molecular regulation and cellular changes in neotenic and metamorphosed axolotls are still poorly investigated. Here, we develop a single-cell sequencing method based on combinatorial hybridization to generate a tissue-based transcriptomic landscape of the neotenic and metamorphosed axolotls. We perform gene expression profiling of over 1 million single cells across 19 tissues to construct the first adult axolotl cell landscape. Comparison of single-cell transcriptomes between the tissues of neotenic and metamorphosed axolotls reveal the heterogeneity of non-immune parenchymal cells in different tissues and established their regulatory network. Furthermore, we describe dynamic gene expression patterns during limb development in neotenic axolotls. This system-level single-cell analysis of molecular characteristics in neotenic and metamorphosed axolotls, serves as a resource to explore the molecular identity of the axolotl and facilitates better understanding of metamorphosis.
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