ArticleProceedings of the National Academy of Sciences of the United States of America2022
Active emulsions in living cell membranes driven by contractile stresses and transbilayer coupling.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Diverse ancestral myosin motors generate and segregate distinct types of nanocluster-rich domains at the plasma membrane.Science advances · 2026Article
- Monte Carlo modeling of the formation and organization of ion channel clustering.PLoS computational biology · 2026Article
- Genome-wide CRISPR screen identifies STK11 as a critical regulator of sialic acid clusters important for influenza A virus attachment.Journal of advanced research · 2026Article
- Flow transports extracellular lipid-anchored proteins across the surface of living COS-7 cells.Biophysical journal · 2026Article
- Flow transports extracellular lipid-anchored proteins across the surface of living COS-7 cells.bioRxiv : the preprint server for biology · 2025Article
- Active transport enables protein condensation in cells.Science advances · 2025Article
- Cell-intrinsic mechanical regulation of plasma membrane accumulation at the cytokinetic furrow.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Cell intrinsic mechanical regulation of plasma membrane accumulation at the cytokinetic furrow.bioRxiv : the preprint server for biology · 2024Article
- The ubiquitous nanocluster: A molecular scale organizing principle that governs cellular information flow.Current opinion in cell biology · 2024Review
- Cell type-specific regulation of CFTR trafficking-on the verge of progress.Frontiers in cell and developmental biology · 2024Review
- The Membrane Phase Transition Gives Rise to Responsive Plasma Membrane Structure and Function.Cold Spring Harbor perspectives in biology · 2023Review
- A Survey of Models of Cell Membranes: Toward a New Understanding of Membrane Organization.Cold Spring Harbor perspectives in biology · 2023Review
- Spontaneous motion of a passive fluid droplet in an active microchannel.Soft matter · 2023Article
- Lipid packing in biological membranes governs protein localization and membrane permeability.Biophysical journal · 2023Article
- Lipid Polarization during Cytokinesis.Cells · 2022Review
- The Importance of the Plasma Membrane in Atherogenesis.Membranes · 2022Review
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Authors and funding
7 authors at 1 institution in 2 countries.
Funding
Abstract
The spatiotemporal organization of proteins and lipids on the cell surface has direct functional consequences for signaling, sorting, and endocytosis. Earlier studies have shown that multiple types of membrane proteins, including transmembrane proteins that have cytoplasmic actin binding capacity and lipid-tethered glycosylphosphatidylinositol-anchored proteins (GPI-APs), form nanoscale clusters driven by active contractile flows generated by the actin cortex. To gain insight into the role of lipids in organizing membrane domains in living cells, we study the molecular interactions that promote the actively generated nanoclusters of GPI-APs and transmembrane proteins. This motivates a theoretical description, wherein a combination of active contractile stresses and transbilayer coupling drives the creation of active emulsions, mesoscale liquid order (lo) domains of the GPI-APs and lipids, at temperatures greater than equilibrium lipid phase segregation. To test these ideas, we use spatial imaging of molecular clustering combined with local membrane order, and we demonstrate that mesoscopic domains enriched in nanoclusters of GPI-APs are maintained by cortical actin activity and transbilayer interactions and exhibit significant lipid order, consistent with predictions of the active composite model.
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