ArticlePloS one2022
L-lactate as an indicator for cellular metabolic status: An easy and cost-effective colorimetric L-lactate assay.
Article in PloS one, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
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- Reduced graphene oxide-incorporated gelatin-oxidized polymannose scaffold-based 3D Lung cancer model for mimicking the tumor microenvironment.Frontiers in oncology · 2026Article
- H3K56 lactylation promotes collagen II synthesis to modulate chondrocyte metabolism in posttraumatic osteoarthritis.Burns & trauma · 2026Article
- Flexible Cu Nanostructured Laser-Induced Graphene Electrodes for Highly Sensitive and Non-Invasive Lactate Detection in Saliva.Biosensors · 2025Article
- 4-Methylumbelliferone Modulates CAIX to Mitigate Hypoxia-Driven Dysregulation and Enhance PD-1 Immunotherapy in Lung Cancer.International journal of molecular sciences · 2025Article
- Characterisation of Titanium-Oxide Thin Films for Efficient pH Sensing in Low-Power Electrochemical Systems.Sensors (Basel, Switzerland) · 2025Article
- Covalent and Site-Specific Immobilization of a Fluorogenic Sensor Protein on Cellulose-Based Paper for Detection of Lactate in Cell Culture Media.Biosensors · 2025Article
- Cytoplasmic physicochemical factors drive malignant transformation by adapting bioenergetic settings.Biochemistry and biophysics reports · 2025Article
- Systematic analysis of the glucose-PTS inApplied and environmental microbiology · 2025Article
- Bacterial Cellulose/Graphene Oxide/Hydroxyapatite Biocomposite: A Scaffold from Sustainable Sources for Bone Tissue Engineering.ACS applied materials & interfaces · 2025Article
- A novel non-invasive sweat lactate biosensor and its application in human exercise monitoring.Frontiers in bioengineering and biotechnology · 2025Article
- MCU-i4, a mitochondrial CaOncology research · 2025Article
- Activity-dependent COX-2 proteolysis modulates aerobic respiration and proliferation in a prostaglandin-independent manner.iScience · 2024Article
- Lactate level as a predictor of outcomes in patients with acute upper gastrointestinal bleeding: A systematic review and meta‑analysis.Experimental and therapeutic medicine · 2024Article
- Viability of HepG2 and MCF-7 cells is not correlated with mitochondrial bioenergetics.Scientific reports · 2023Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn recent times, the study of metabolic pathways has become inevitable and predominant for a variety of research fields as cancer biology and immunology. L-lactate as a product of anaerobic glycolysis has shown to be an important indicator of the cellular metabolic status and can be associated with diverse cellular effects. For this reason, L-lactate assay kits are of high demand when metabolic effects need to be considered. Nevertheless, commercially available kits are not affordable if multiple samples must be evaluated. PRINCIPAL FINDING: In this work, we develop an easy and cost-effective colorimetric assay for quantification of L-lactate suitable for cells with low or high L-lactate production based on LDH activity and suitable for 96 well-plate format. Using different metabolic regulators, we demonstrate the capacity of the assay to detect and quantify L-lactate from the supernatant of HeLa cancer cell line. Furthermore, we validate the assay against a commercially available kit by demonstrating no significant difference between both assays. Finally, we show that the assay is capable of quantifying L-lactate in primary cells such as hPBMCs that were stimulated with toll-like receptor ligands and treated with different metabolic regulators.
conclusionWe herein present an easy custom assay that is suitable for cells with low and high L-lactate production at very low cost compared to commercially available kits. These advantages of the custom assay can simplify the research in the field of metabolism and related fields.
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