Evidence map›Paper›PMID 35867400›Full record

ArticleMicrobiology spectrum2022

p53 Binding Sites in Long Terminal Repeat 5Hs (LTR5Hs) of Human Endogenous Retrovirus K Family (HML-2 Subgroup) Play Important Roles in the Regulation of LTR5Hs Transcriptional Activity.

Mengying Liu, Lei Jia, Hanping Li, Yongjian Liu, Jingwan Han, Xiaolin Wang, Tianyi Li, Jingyun Li, Bohan Zhang, Xiuli Zhai and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
2.2field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 29 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Review
  5. Review
  6. Review
  7. Article
  8. The role of HERV envelope protein in ovarian cancer.Frontiers in cell and developmental biology · 2025
    Review
  9. Review
  10. Article
  11. Article
  12. Review
  13. Long COVID as a Tauopathy: Of "Brain Fog" and "Fusogen Storms".International journal of molecular sciences · 2023
    Review
  14. Article
  15. Review
  16. Review
  17. Article
  18. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Mengying Liu *College of Life Science and Technology, Beijing University of Chemical Technologygrid.48166.3d, Beijing, China.
Lei Jia *Department of Virology, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
Hanping LiDepartment of Virology, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
Yongjian LiuDepartment of Virology, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
Jingwan HanDepartment of Virology, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
Xiaolin WangDepartment of Virology, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
Tianyi LiDepartment of Virology, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
Jingyun LiDepartment of Virology, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
Bohan ZhangDepartment of Virology, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
Xiuli ZhaiDepartment of Virology, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
Changyuan YuCollege of Life Science and Technology, Beijing University of Chemical Technologygrid.48166.3d, Beijing, China.
Lin LiDepartment of Virology, Beijing Institute of Microbiology and Epidemiology, Beijing, China.ORCID 0000-0001-6124-5237
Institute of Microbiology · CNBeijing University of Chemical Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The long terminal repeats (LTRs) of human endogenous retroviruses (HERVs) are distributed throughout the human genome and provide favorable conditions to regulate the expression of their adjacent genes. HML-2 is the most biologically active subgroup of the HERV-K family, and expression of its members has been associated with many cancer types. The LTRs of HML-2 have been classified into three subgroups (LTR5A, LTR5B, and LTR5Hs) based on phylogenetic analyses. The current study aimed to explore the LTR transcriptional activity differences among the three subtypes and further explore the underlying factors. A total of 43 LTR5A elements, 62 LTR5B elements, and 194 LTR5Hs elements were selected. A phylogenetic tree showed that the LTR5Hs group was clearly separated from the LTR5A and LTR5B groups. A luciferase reporter assay indicated that LTR5Hs had the strongest promoter activity, followed by LTR5A and LTR5B. To investigate the underlying factors, LTR5Hs was divided into 4 sections, and the homologous fragments in LTR5B were replaced successively. Replacement of the third section (-263 to 0) significantly increased LTR5B activity. Subsequent mutation experiments revealed that the increased transcriptional activity was induced by the TATA box and the two p53 binding sites within the section. Further interference with

Indexed as

Endogenous RetrovirusesBinding SitesHumansMalePhylogenyTerminal Repeat SequencesTumor Suppressor Protein p53TP53 protein, humanTumor Suppressor Protein p53HERVHML-2LTR5Hsp53transcriptional activity

Identifiers

PMID35867400
PMCPMC9430305
OpenAlexW4285728907

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.