ArticleMethods in molecular biology (Clifton, N.J.)2022
High-Throughput DNA FISH (hiFISH).
Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Review
- High-throughput image processing software for the study of nuclear architecture and gene expression.Scientific reports · 2024Article
- From feulgen to modern methods: marking a century of DNA imaging advances.Histochemistry and cell biology · 2024Review
- Allele-level visualization of transcription and chromatin by high-throughput imaging.Histochemistry and cell biology · 2024Article
- Allele-level visualization of transcription and chromatin by high-throughput imaging.Research square · 2024Article
- Allele-level visualization of transcription and chromatin by high-throughput imaging.bioRxiv : the preprint server for biology · 2024Article
- The New Era of Cancer Cytogenetics and Cytogenomics.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Article
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3 authors.
Funding
Abstract
High-throughput DNA fluorescence in situ hybridization (hiFISH) combines multicolor combinatorial DNA FISH staining with automated image acquisition and analysis to visualize and localize tens to hundreds of genomic loci in up to millions of cells. hiFISH can be used to measure physical distances between pairs of genomic loci, radial distances from genomic loci to the nuclear edge or center, and distances between genomic loci and nuclear structures defined by protein or RNA markers. The resulting large datasets of 3D spatial distances can be used to study cellular heterogeneity in genome architecture and the molecular mechanisms underlying this phenomenon in a variety of cellular systems. In this chapter we provide detailed protocols for hiFISH to measure distances between genomic loci, including all steps involved in DNA FISH probe design and preparation, cell culture, DNA FISH staining in 384-well imaging plates, automated image acquisition and analysis, and, finally, statistical analysis.
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