Evidence map›Paper›PMID 35867173›Full record

ArticleAntonie van Leeuwenhoek2022

AcdR protein is an activator of transcription of 1-aminocyclopropane-1-carboxylate deaminase in Methylobacterium radiotolerans JCM 2831.

Galina A Ekimova, Dmitry N Fedorov, Nina V Doronina, Valentina N Khmelenina, Ildar I Mustakhimov

Abstract read
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In one paragraph

Article in Antonie van Leeuwenhoek, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Galina A EkimovaG.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of the Russian Academy of Sciences (IBPM RAS), Federal Research Center Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Pushchino, Moscow Region, Russia, 142290. ekimovag22@gmail.com.
Dmitry N FedorovG.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of the Russian Academy of Sciences (IBPM RAS), Federal Research Center Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Pushchino, Moscow Region, Russia, 142290.
Nina V DoroninaG.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of the Russian Academy of Sciences (IBPM RAS), Federal Research Center Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Pushchino, Moscow Region, Russia, 142290.
Valentina N KhmeleninaG.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of the Russian Academy of Sciences (IBPM RAS), Federal Research Center Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Pushchino, Moscow Region, Russia, 142290.
Ildar I MustakhimovG.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of the Russian Academy of Sciences (IBPM RAS), Federal Research Center Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Pushchino, Moscow Region, Russia, 142290.
G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms · RU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

It has been previously shown that a number of plant associated methylotrophic bacteria contain an enzyme aminocyclopropane carboxylate (ACC) deaminase (AcdS) hydrolyzing ACC, the immediate precursor of ethylene in plants. The genome of the epiphytic methylotroph Methylobacterium radiotolerans JCM2831 contains an open reading frame encoding a protein homologous to transcriptional regulatory protein AcdR of the Lrp (leucine-responsive regulatory protein) family. The acdR gene of M. radiotolerans was heterologously expressed in Escherichia coli and purified. The results of gel retardation experiments have shown that AcdR specifically binds the DNA fragment containing the promoter-operator region of the acdS gene. ACC decreased electrophoretic mobility of the AcdR-DNA complex whereas leucine had no effect on the complex mobility. The mutant strains of M. radiotolerans obtained by insertion of a tetracycline cassette in the acdS or acdR gene lost the ACC-deaminase activity but the strains with complementation of the mutation recovered this function. The acdS

Indexed as

Carbon-Carbon LyasesMethylobacteriumEscherichia coliPromoter Regions, GeneticTranscription, Genetic1-aminocyclopropane-1-carboxylate deaminaseCarbon-Carbon Lyases1-aminocyclopropane-1-carboxylate deaminaseMethylobacteriumRegulation of transcription

Identifiers

PMID35867173
OpenAlexW4286698133

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.