ArticleeLife2022
A viral toolbox for conditional and transneuronal gene expression in zebrafish.
Article in eLife, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- An applicable and efficient retrograde monosynaptic circuit mapping tool for larval zebrafish.eLife · 2026Article
- Article
- Trans-Synaptic Virus Tracing Reveals Reciprocal Connections of the Interpeduncular Nucleus With the Mood, Exteroceptive, Interoceptive, and Motor Control Systems.The Journal of comparative neurology · 2025Article
- Adeno-associated viruses for efficient gene expression in the axolotl nervous system.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Transsynaptic labeling and transcriptional control of zebrafish neural circuits.Nature neuroscience · 2025Article
- Base editors in zebrafish: a new era for functional genomics and disease modeling.Frontiers in genome editing · 2025Review
- Third-generation rabies viral vectors allow nontoxic retrograde targeting of projection neurons with greatly increased efficiency.Cell reports methods · 2023Article
- Zebrafish brain atlases: a collective effort for a tiny vertebrate brain.Neurophotonics · 2023Article
- A new microfluidic model to study dendritic remodeling and mitochondrial dynamics during axonal regeneration of adult zebrafish retinal neurons.Frontiers in molecular neuroscience · 2023Article
- A minimally invasive fin scratching protocol for fast genotyping and early selection of zebrafish embryos.Scientific reports · 2022Article
- Zebrafish behavior as a gateway to nervous system assembly and plasticity.Development (Cambridge, England) · 2022Article
- Cre-Dependent Anterograde Transsynaptic Labeling and Functional Imaging in Zebrafish Using VSV With Reduced Cytotoxicity.Frontiers in neuroanatomy · 2021Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
The zebrafish is an important model in systems neuroscience but viral tools to dissect the structure and function of neuronal circuitry are not established. We developed methods for efficient gene transfer and retrograde tracing in adult and larval zebrafish by herpes simplex viruses (HSV1). HSV1 was combined with the Gal4/UAS system to target cell types with high spatial, temporal, and molecular specificity. We also established methods for efficient transneuronal tracing by modified rabies viruses in zebrafish. We demonstrate that HSV1 and rabies viruses can be used to visualize and manipulate genetically or anatomically identified neurons within and across different brain areas of adult and larval zebrafish. An expandable library of viruses is provided to express fluorescent proteins, calcium indicators, optogenetic probes, toxins and other molecular tools. This toolbox creates new opportunities to interrogate neuronal circuits in zebrafish through combinations of genetic and viral approaches.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.