Evidence map›Paper›PMID 35866602›Full record

ArticleActa biochimica et biophysica Sinica2022

Parthenolide reveals an allosteric mode to inhibit the deISGylation activity of SARS-CoV‑2 papain-like protease.

Zhihui Zou, Huizhuang Shan, Demeng Sun, Li Xia, Yulong Shi, Jiahui Wan, Aiwu Zhou, Yunzhao Wu, Hanzhang Xu, Hu Lei and 2 more

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta biochimica et biophysica Sinica, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Pharmaceuticals (Basel, Switzerland) · 2026
    Article
  2. Review
  3. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Zhihui ZouHongqiao International Institute of Medicine, Shanghai Tongren Hospital / Faculty of Basic Medicine, Chemical Biology Division of Shanghai Universities E-Institutes, Key Laboratory of Cell Differentiation and Apoptosis of the Chinese Ministry of Education, Chinese Academy of Medical Sciences Research Unit 2019RU043, Shanghai Jiao Tong University School of Medicine (SJTU-SM), Shanghai 200025, China.
Huizhuang ShanHongqiao International Institute of Medicine, Shanghai Tongren Hospital / Faculty of Basic Medicine, Chemical Biology Division of Shanghai Universities E-Institutes, Key Laboratory of Cell Differentiation and Apoptosis of the Chinese Ministry of Education, Chinese Academy of Medical Sciences Research Unit 2019RU043, Shanghai Jiao Tong University School of Medicine (SJTU-SM), Shanghai 200025, China.
Demeng SunTsinghua-Peking Center for Life Sciences, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Beijing Advanced Innovation Center for Structural Biology, Department of Chemistry, Tsinghua University, Beijing 100084, China.
Li XiaHongqiao International Institute of Medicine, Shanghai Tongren Hospital / Faculty of Basic Medicine, Chemical Biology Division of Shanghai Universities E-Institutes, Key Laboratory of Cell Differentiation and Apoptosis of the Chinese Ministry of Education, Chinese Academy of Medical Sciences Research Unit 2019RU043, Shanghai Jiao Tong University School of Medicine (SJTU-SM), Shanghai 200025, China.
Yulong ShiCAS Key Laboratory of Receptor Research, Drug Discovery and Design Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Jiahui WanHongqiao International Institute of Medicine, Shanghai Tongren Hospital / Faculty of Basic Medicine, Chemical Biology Division of Shanghai Universities E-Institutes, Key Laboratory of Cell Differentiation and Apoptosis of the Chinese Ministry of Education, Chinese Academy of Medical Sciences Research Unit 2019RU043, Shanghai Jiao Tong University School of Medicine (SJTU-SM), Shanghai 200025, China.
Aiwu ZhouHongqiao International Institute of Medicine, Shanghai Tongren Hospital / Faculty of Basic Medicine, Chemical Biology Division of Shanghai Universities E-Institutes, Key Laboratory of Cell Differentiation and Apoptosis of the Chinese Ministry of Education, Chinese Academy of Medical Sciences Research Unit 2019RU043, Shanghai Jiao Tong University School of Medicine (SJTU-SM), Shanghai 200025, China.
Yunzhao WuHongqiao International Institute of Medicine, Shanghai Tongren Hospital / Faculty of Basic Medicine, Chemical Biology Division of Shanghai Universities E-Institutes, Key Laboratory of Cell Differentiation and Apoptosis of the Chinese Ministry of Education, Chinese Academy of Medical Sciences Research Unit 2019RU043, Shanghai Jiao Tong University School of Medicine (SJTU-SM), Shanghai 200025, China.
Hanzhang XuHongqiao International Institute of Medicine, Shanghai Tongren Hospital / Faculty of Basic Medicine, Chemical Biology Division of Shanghai Universities E-Institutes, Key Laboratory of Cell Differentiation and Apoptosis of the Chinese Ministry of Education, Chinese Academy of Medical Sciences Research Unit 2019RU043, Shanghai Jiao Tong University School of Medicine (SJTU-SM), Shanghai 200025, China.
Hu LeiHongqiao International Institute of Medicine, Shanghai Tongren Hospital / Faculty of Basic Medicine, Chemical Biology Division of Shanghai Universities E-Institutes, Key Laboratory of Cell Differentiation and Apoptosis of the Chinese Ministry of Education, Chinese Academy of Medical Sciences Research Unit 2019RU043, Shanghai Jiao Tong University School of Medicine (SJTU-SM), Shanghai 200025, China.
Zhijian XuCAS Key Laboratory of Receptor Research, Drug Discovery and Design Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Yingli WuHongqiao International Institute of Medicine, Shanghai Tongren Hospital / Faculty of Basic Medicine, Chemical Biology Division of Shanghai Universities E-Institutes, Key Laboratory of Cell Differentiation and Apoptosis of the Chinese Ministry of Education, Chinese Academy of Medical Sciences Research Unit 2019RU043, Shanghai Jiao Tong University School of Medicine (SJTU-SM), Shanghai 200025, China.
Chinese Academy of Sciences · CNCenter for Life Sciences · CNGuangdong Academy of Medical Sciences · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The coronavirus papain-like protease (PLpro) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for viral polypeptide cleavage and the deISGylation of interferon-stimulated gene 15 (ISG15), which enable it to participate in virus replication and host innate immune pathways. Therefore, PLpro is considered an attractive antiviral drug target. Here, we show that parthenolide, a germacrane sesquiterpene lactone, has SARS-CoV-2 PLpro inhibitory activity. Parthenolide covalently binds to Cys-191 or Cys-194 of the PLpro protein, but not the Cys-111 at the PLpro catalytic site. Mutation of Cys-191 or Cys-194 reduces the activity of PLpro. Molecular docking studies show that parthenolide may also form hydrogen bonds with Lys-192, Thr-193, and Gln-231. Furthermore, parthenolide inhibits the deISGylation but not the deubiquitinating activity of PLpro

Indexed as

Coronavirus Papain-Like ProteasesCOVID-19 Drug TreatmentAntiviral AgentsHumansInterferonsLactonesMolecular Docking SimulationPapainPeptide HydrolasesSARS-CoV-2SesquiterpenesSesquiterpenes, GermacraneUbiquitinAntiviral AgentsCoronavirus Papain-Like ProteasesInterferonsLactonesPapainpapain-like protease, SARS-CoV-2parthenolidePeptide HydrolasesSesquiterpenesSesquiterpenes, GermacraneUbiquitinCOVID-19deISGylationParthenolidePLproSARS-CoV-2

Identifiers

PMID35866602
PMCPMC9827819
OpenAlexW4286250242

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.