ArticleAdvanced healthcare materials2022
Cell-Laden Gradient Microgel Suspensions for Spatial Control of Differentiation During Biofabrication.
Article in Advanced healthcare materials, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- Photoresponsive Granular Hydrogels Enable Spatiotemporal Control of Matrix Mechanics and MSC Behavior.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Packed Hydrogel Microfibers as Scaffolds Supporting Dynamic Cellular Behavior and Biomaterial Inks in 3D Printing.Advanced healthcare materials · 2026Article
- Tuning Hydrogel Mechanics and Microstructure to Maximize Extracellular Vesicle Production from Mesenchymal Stem Cells.Cellular and molecular bioengineering · 2026Article
- Microengineered Gradient Hydrogels for Mechanobiology.Advanced healthcare materials · 2026Review
- A biofabricated 3D cancer-stroma tumor microenvironment model.Biofabrication · 2025Article
- Practical Guide to the Design of Granular Hydrogels for Customizing Complex Cellular Microenvironments.Advanced healthcare materials · 2025Review
- Bioprinted Scaffolds for Biomimetic Applications: A State-of-the-Art Technology.Biomimetics (Basel, Switzerland) · 2025Review
- Porous granular hydrogel scaffolds biofabricated from dual-crosslinked hydrogel microparticles for breast tissue engineering.Materials today. Bio · 2025Article
- Micro-fragmented collagen hydrogel wound dressing: Enhanced porosity facilitates elevated stem cell survival and paracrine effects for accelerated wound maturation.Materials today. Bio · 2025Article
- Multi-material Volumetric Bioprinting and Plug-and-play Suspension Bath Biofabrication via Bioresin Molecular Weight Tuning and via Multiwavelength Alignment Optics.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Three-Dimensional Printed Cell-Adaptable Nanocolloidal Hydrogel Induces Endogenous Osteogenesis for Bone Repair.Biomaterials research · 2025Article
- Engineering Microgel Packing to Tailor the Physical and Biological Properties of Gelatin Methacryloyl Granular Hydrogel Scaffolds.Advanced healthcare materials · 2024Article
- Using Chemistry To Recreate the Complexity of the Extracellular Matrix: Guidelines for Supramolecular Hydrogel-Cell Interactions.Journal of the American Chemical Society · 2024Review
- 4D bioprinting of programmed dynamic tissues.Bioactive materials · 2024Review
- 3D Volumetric Mechanosensation of MCF7 Breast Cancer Spheroids in a Linear Stiffness Gradient GelAGE.Advanced healthcare materials · 2023Article
- Gas-modulating microcapsules for spatiotemporal control of hypoxia.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Tunable Mesoscopic Collagen Island Architectures Modulate Stem Cell Behavior.Advanced materials (Deerfield Beach, Fla.) · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
During tissue development, stem and progenitor cells form functional tissue with high cellular diversity and intricate micro- and macro-architecture. Current approaches have attempted to replicate this process with materials cues or through spontaneous cell self-organization. However, cell-directed and materials-directed organization are required simultaneously to achieve biomimetic structure and function. Here, it is shown how integrating live adipose derived stem cells with gradient microgel suspensions steers divergent differentiation outcomes. Microgel matrices composed of small particles are found to promote adipogenic differentiation, while larger particles fostered increased cell spreading and osteogenic differentiation. Tuning the matrix formulation demonstrates that early cell adhesion and spreading dictate differentiation outcome. Combining small and large microgels into gradients spatially directs proliferation and differentiation over time. After 21 days of culture, osteogenic conditions foster significant mineralization within the individual microgels, thereby providing cell-directed changes in composition and mechanics within the gradient porous scaffold. Freeform printing of high-density cell suspensions is performed across these gradients to demonstrate the potential for hierarchical tissue biofabrication. Interstitial porosity influences cell expansion from the print and microgel size guides spatial differentiation, thereby providing scope to fabricate tissue gradients at multiple scales through integrated and printed cell populations.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.