ArticleiScience2022
Ontogeny of cellular organization and LGR5 expression in porcine cochlea revealed using tissue clearing and 3D imaging.
Article in iScience, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 11 citations in OpenAlex.
- Biodistribution and transport of intratympanically administered drugs in the inner ear using a porcine ex-vivo chamber model.Materials & design · 2026Article
- Spiner, deep learning-based automated detection of spiral ganglion neurons in intact cochleae.iScience · 2025Article
- A gene edited pig model for studying LGR5Frontiers in genome editing · 2024Review
- Surgical procedure of intratympanic injection and inner ear pharmacokinetics simulation in domestic pigs.Frontiers in pharmacology · 2024Article
- Intelligent Beam Optimization for Light-Sheet Fluorescence Microscopy through Deep Learning.Intelligent computing (Washington, D.C.) · 2024Article
- T-CLEARE: a pilot community-driven tissue clearing protocol repository.Frontiers in bioengineering and biotechnology · 2024Article
- Enhancing Light-Sheet Fluorescence Microscopy Illumination Beams through Deep Design Optimization.bioRxiv : the preprint server for biology · 2023Article
- Three-dimensional mouse cochlea imaging based on the modified Sca/eS using confocal microscopy.Anatomical science international · 2023Article
- COMBINe enables automated detection and classification of neurons and astrocytes in tissue-cleared mouse brains.Cell reports methods · 2023Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Over 11% of the world's population experience hearing loss. Although there are promising studies to restore hearing in rodent models, the size, ontogeny, genetics, and frequency range of hearing of most rodents' cochlea do not match that of humans. The porcine cochlea can bridge this gap as it shares many anatomical, physiological, and genetic similarities with its human counterpart. Here, we provide a detailed methodology to process and image the porcine cochlea in 3D using tissue clearing and light-sheet microscopy. The resulting 3D images can be employed to compare cochleae across different ages and conditions, investigate the ontogeny of cochlear cytoarchitecture, and produce quantitative expression maps of LGR5, a marker of cochlear progenitors in mice. These data reveal that hair cell organization, inner ear morphology, cellular cartography in the organ of Corti, and spatiotemporal expression of LGR5 are dynamic over developmental stages in a pattern not previously documented.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.