Evidence map›Paper›PMID 35863040›Full record

ArticleMicrobiology spectrum2022

Single-Cell Analysis of the Antimicrobial and Bactericidal Activities of the Antimicrobial Peptide Magainin 2.

Farzana Hossain, Md Masum Billah, Masahito Yamazaki

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Farzana HossainNanomaterials Research Division, Research Institute of Electronics, Shizuoka Universitygrid.263536.7, Shizuoka, Japan.
Md Masum BillahIntegrated Bioscience Section, Graduate School of Science and Technology, Shizuoka Universitygrid.263536.7, Shizuoka, Japan.
Masahito YamazakiNanomaterials Research Division, Research Institute of Electronics, Shizuoka Universitygrid.263536.7, Shizuoka, Japan.ORCID 0000-0002-5959-9914
Shizuoka University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Antimicrobial peptides (AMPs) inhibit the proliferation of or kill bacterial cells. To measure these activities, several methods have been used, which provide only the average value of many cells. Here, we report the development of a method to examine the antimicrobial and bactericidal activities of AMPs at the single-cell level (i.e., single-cell analysis) and apply this strategy to examine the interaction of an AMP, magainin 2 (Mag), with Escherichia coli cells. Using this method, we monitored the proliferation of single cells on agar in a microchamber and measured the distribution of the number of cells in each microcolony using optical microscopy. For method A, we incubated cells in the presence of various concentrations of AMPs for 3 h. The fraction of microcolonies containing only a single cell,

Indexed as

Anti-Infective AgentsAntimicrobial PeptidesAdenosine MonophosphateAgarAnti-Bacterial AgentsEscherichia coliMagaininsMicrobial Sensitivity TestsSingle-Cell AnalysisAdenosine MonophosphateAgarAnti-Bacterial AgentsAnti-Infective AgentsAntimicrobial PeptidesMagaininsantimicrobial peptidesbactericidal activitycell deathcell permeabilizationcell proliferationconfocal microscopeEscherichia coliinteraction timemicrocolonyplasma membranesingle-cell analysis

Identifiers

PMID35863040
PMCPMC9431230
OpenAlexW4285084116

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.