Evidence map›Paper›PMID 35862665›Full record

ArticleApplied and environmental microbiology2022

Exploiting the Type I-B CRISPR Genome Editing System in Thermoanaerobacterium aotearoense SCUT27 and Engineering the Strain for Enhanced Ethanol Production.

Kaiqun Dai, Hongxin Fu, Xiaolong Guo, Chunyun Qu, Yang Lan, Jufang Wang

Open access · greenAbstract read
In one paragraph

Article in Applied and environmental microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.6field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 20 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Review
  7. A fluorescent reporter system for anaerobic thermophiles.Frontiers in bioengineering and biotechnology · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Kaiqun DaiSchool of Biology and Biological Engineering, South China University of Technologygrid.79703.3a, Guangzhou, China.
Hongxin FuSchool of Biology and Biological Engineering, South China University of Technologygrid.79703.3a, Guangzhou, China.
Xiaolong GuoSchool of Biology and Biological Engineering, South China University of Technologygrid.79703.3a, Guangzhou, China.
Chunyun QuSchool of Biology and Biological Engineering, South China University of Technologygrid.79703.3a, Guangzhou, China.
Yang LanSchool of Biology and Biological Engineering, South China University of Technologygrid.79703.3a, Guangzhou, China.
Jufang WangSchool of Biology and Biological Engineering, South China University of Technologygrid.79703.3a, Guangzhou, China.ORCID 0000-0002-9908-1001
South China University of Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Thermoanaerobacterium aotearoense strain SCUT27 is a potential industrial biofuel-producing strain because of its broad substrate spectrum, especially the ability to co-use glucose and xylose. The bottleneck hindering the development of strain SCUT27 is the lack of selective markers for polygene manipulation in this thermophilic bacterium. In this study, the endogenous type I-B clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system was developed for multiplex genome editing of strain SCUT27. The protospacer-adjacent motif was identified by

Indexed as

Gene EditingThermoanaerobacteriumBiofuelsCRISPR-Cas SystemsEthanolXyloseBiofuelsEthanolXylosebioethanolgenome editingThermoanaerobacterium aotearoensetype I-B CRISPR

Identifiers

PMID35862665
PMCPMC9361813
OpenAlexW4285037667

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.