Evidence map›Paper›PMID 35862251›Full record

ArticleInternational journal of immunogenetics2022

A susceptibility putative haplotype within NLRP3 inflammasome gene influences ischaemic stroke risk in the population of Punjab, India.

Nitin Kumar, Manminder Kaur, Gurjinderpal Singh, Srishti Valecha, Rubanpal Khinda, Mario Di Napoli, Monica Singh, Puneetpal Singh, Sarabjit Mastana

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of immunogenetics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.6field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 3 countries.

Nitin KumarDivision of Molecular Genetics, Department of Human Genetics, Punjabi University, Patiala, Punjab, India.
Manminder KaurDepartment of Neurology, MK Neuro Centre, Patiala, Punjab, India.
Gurjinderpal SinghDepartment of Neurology, Bhatia Hospital Neuro and Multispecialty, Patiala, Punjab, India.
Srishti ValechaDivision of Molecular Genetics, Department of Human Genetics, Punjabi University, Patiala, Punjab, India.
Rubanpal KhindaDivision of Molecular Genetics, Department of Human Genetics, Punjabi University, Patiala, Punjab, India.
Mario Di NapoliDepartment of Neurological Service, Annunziata Hospital, Sulmona, L'Aquila, Italy.
Monica SinghDivision of Molecular Genetics, Department of Human Genetics, Punjabi University, Patiala, Punjab, India.
Puneetpal SinghDivision of Molecular Genetics, Department of Human Genetics, Punjabi University, Patiala, Punjab, India.
Sarabjit MastanaHuman Genomics Laboratory, School of Sport, Exercise and Health Sciences, Loughborough University, Loughborough, UK.ORCID https://orcid.org/0000-0002-9553-4886
Punjabi University · INBhatia Hospital · INGovernment Medical College · INLoughborough University · GBOspedale SS. Annunziata · IT

Funding

Council of Scientific and Industrial Research 09/140(0174)/2018-EMR-1
6 · The paper itself

Abstract

Despite strong genetic implications of NLRP3 inflammasome, its examination as genetic determinant of ischaemic stroke (IS) remains to be done in Punjab, which has been investigated in this study. In this case control study, 400 subjects (200 IS patients, 200 stroke free controls) were included. Contributions of 5 single nucleotide polymorphisms (SNPs) including a functional SNP within NLRP3 gene (rs10754558, rs4612666, rs2027432, rs3738488 and rs1539019) for the risk of IS were investigated through genetic models after correcting the effect of significant variables. Plasma levels of three pro-inflammatory markers, that is, C-reactive protein (CRP), interleukin-1beta (IL-1β) and interleukin-18 (IL-18) were measured by enzyme-linked immunosorbent assays (ELISA). Minor alleles of 3 out of 5 SNPs (rs10754558, rs4612666 and rs1539019) exhibited association with IS risk in additive, recessive and multiplicative models. Multivariable regression analysis confirmed that higher levels of systolic blood pressure (β ± SE: 1.42 ± 0.57, p = .013), CRP (β ± SE: 1.22 ± 0.41, p = .003), IL-1β (β ± SE: 1.78 ± 0.88, p = .043) and IL-18 (β ± SE: 1.13 ± 0.49, p = .021) were independent risk predictors for IS. Haplotype analysis revealed a susceptibility putative haplotype GTGTA, which approximately doubled the IS risk (OR: 1.98, 95% CI: 1.12-3.78, p = .04) in dominant mode after adjusting the effect with confounding variables. This susceptibility putative haplotype GTGTA was significantly associated with increased concentrations of CRP (β = 1.21, p = .014) and IL-1β (β = 1.53, p = .034) in dose-dependent manner (less in carriers of 1 copy than those who had 2 copies of GTGTA). The present study has revealed a susceptibility putative haplotype GTGTA within NLRP3 gene, carriers of which have double the risk of IS by having increased plasma levels of CRP and IL-1β in a dose-dependent manner.

Indexed as

Brain IschemiaIschemic StrokeStrokeCase-Control StudiesGenetic Predisposition to DiseaseHaplotypesHumansInflammasomesInterleukin-18Interleukin-1betaNLR Family, Pyrin Domain-Containing 3 ProteinPolymorphism, Single NucleotideInflammasomesInterleukin-18Interleukin-1betaNLR Family, Pyrin Domain-Containing 3 Proteindose-dependentIndiaischaemic strokeNLRP3 gene polymorphismpro-inflammatory variablesrisk predictors

Identifiers

PMID35862251
PMCPMC9546049
OpenAlexW4286203189

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.