ArticleThe Plant cell2022
The phospholipid flippase ALA3 regulates pollen tube growth and guidance in Arabidopsis.
Article in The Plant cell, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- Functional Characterization ofBiology · 2026Article
- OsPRK1/2/3-mediated reactive oxygen species signaling is required for pollen tube germination in rice.Journal of integrative plant biology · 2025Article
- Novel small molecules disrupting polarized cell expansion and development in the moss,Plant biotechnology (Tokyo, Japan) · 2025Article
- Article
- Genome-wide identification of the P4ATPase gene family and its response to biotic and abiotic stress in soybean (Glycine max L.).BMC genomics · 2025Article
- Decoding small peptides: Regulators of plant growth and stress resilience.Journal of integrative plant biology · 2025Review
- GhCASPL1 regulates secondary cell wall thickening in cotton fibers by stabilizing the cellulose synthase complex on the plasma membrane.Journal of integrative plant biology · 2024Article
- The Role of Female and Male Genes in Regulating Pollen Tube Guidance in Flowering Plants.Genes · 2024Review
- Exploring lipid-protein interactions in plant membranes.Journal of experimental botany · 2024Review
- Arabidopsis class A S-acyl transferases modify the pollen receptors LIP1 and PRK1 to regulate pollen tube guidance.The Plant cell · 2024Article
- Small Peptides: Orchestrators of Plant Growth and Developmental Processes.International journal of molecular sciences · 2024Review
- Flipping the script: Advances in understanding how and why P4-ATPases flip lipid across membranes.Biochimica et biophysica acta. Molecular cell research · 2024Review
- Evolution of the thermostability of actin-depolymerizing factors enhances the adaptation of pollen germination to high temperature.The Plant cell · 2024Article
- Lineage-specific gene duplication and expansion of DUF1216 gene family in Brassicaceae.PloS one · 2024Article
- Polar Localization Analysis of Anionic Phospholipids and Their Binding Proteins in Arabidopsis Pollen Tubes.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Characterization of pollen tube development in distant hybridization of Chinese cork oak (Quercus variabilis L.).Planta · 2023Article
- Arabidopsis flippase ALA3 is required for adjustment of early subcellular trafficking in plant response to osmotic stress.Journal of experimental botany · 2023Article
- Mini-review: Lipid flippases as putative targets for biotechnological crop improvement.Frontiers in plant science · 2023Review
- ALA3 regulates pollen tube growth and guidance by keeping PRKs in all the right places.The Plant cell · 2022Article
- Deficiencies in cluster-2 ALA lipid flippases result in salicylic acid-dependent growth reductions.Physiologia plantarumArticle
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10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pollen tube guidance regulates the growth direction and ovule targeting of pollen tubes in pistils, which is crucial for the completion of sexual reproduction in flowering plants. The Arabidopsis (Arabidopsis thaliana) pollen-specific receptor kinase (PRK) family members PRK3 and PRK6 are specifically tip-localized and essential for pollen tube growth and guidance. However, the mechanisms controlling the polar localization of PRKs at the pollen tube tip are unclear. The Arabidopsis P4-ATPase ALA3 helps establish the polar localization of apical phosphatidylserine (PS) in pollen tubes. Here, we discovered that loss of ALA3 function caused pollen tube defects in growth and ovule targeting and significantly affected the polar localization pattern of PRK3 and PRK6. Both PRK3 and PRK6 contain two polybasic clusters in the intracellular juxtamembrane domain, and they bound to PS in vitro. PRK3 and PRK6 with polybasic cluster mutations showed reduced or abolished binding to PS and altered polar localization patterns, and they failed to effectively complement the pollen tube-related phenotypes of prk mutants. These results suggest that ALA3 influences the precise localization of PRK3, PRK6, and other PRKs by regulating the distribution of PS, which plays a key role in regulating pollen tube growth and guidance.
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