Evidence map›Paper›PMID 35859785›Full record

ArticleData in brief2022

Dataset of 16S rRNA and alkB genes in hydrocarbon polluted soils of Kuwait as revealed by Pyrosequencing.

Sabah Al-Momin, Nazima Habibi, Rita Rahmeh

Abstract read
In one paragraph

Article in Data in brief, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Sabah Al-MominBiotechnology Program, Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, PO Box 24885 Safat, 13109, Kuwait.
Nazima HabibiBiotechnology Program, Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, PO Box 24885 Safat, 13109, Kuwait.
Rita RahmehBiotechnology Program, Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, PO Box 24885 Safat, 13109, Kuwait.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The data in this article was generated by high throughput sequencing of moderately hydrocarbon polluted sites (S1 and S2) and a heavily polluted site (S3) in Kuwait. Deoxyribonucleic acid (DNA) extracted from each site was subjected to polymerase chain reaction (PCR) amplification employing conserved primers of 16S rRNA and alkB genes. Unique Molecular Identifiers (MID) tags were added to individual samples prior to pooling and sequencing on a Roche GS FLX platform using Pyrosequencing Titanium Chemistry. Raw sff files were deposited to the public repository of National Centre for Biotechnology Information (NCBI) under accession no PRJNA816075. The sff files were clipped according to the MID tags and converted to fasta format. 16S rRNA gene sequences were aligned against the SILVA database. The predominant genera at S1 and S2 was

Indexed as

BioremediationContaminated soilHydrocarbon degrading bacteriaNext generation sequencing

Identifiers

PMID35859785
PMCPMC9289844

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.