Evidence map›Paper›PMID 35859064›Full record

ArticleCurrent microbiology2022

Chlamydia trachomatis L2/434/Bu Favors Hypoxia for its Growth in Human Lymphoid Jurkat Cells While Maintaining Production of Proinflammatory Cytokines.

Ryoya Tsujikawa, Jeewan Thapa, Torahiko Okubo, Shinji Nakamura, Saicheng Zhang, Yoshikazu Furuta, Hideaki Higashi, Hiroyuki Yamaguchi

Abstract read
PubMed Publisher
In one paragraph

Article in Current microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 91% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Ryoya TsujikawaDepartment of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Kita 12, Nishi 5, Kitaku, Sapporo, Hokkaido, 060-0812, Japan.
Jeewan ThapaDivision of Bioresources, International Institute for Zoonosis Control, Hokkaido University, North-20, West-10, Kita-ku, Sapporo, 001-0020, Japan.
Torahiko OkuboDepartment of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Kita 12, Nishi 5, Kitaku, Sapporo, Hokkaido, 060-0812, Japan.
Shinji NakamuraDivision of Biomedical Imaging Research, and Division of Ultrastructural Research, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
Saicheng ZhangDepartment of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Kita 12, Nishi 5, Kitaku, Sapporo, Hokkaido, 060-0812, Japan.
Yoshikazu FurutaDivision of Infection and Immunity, International Institute for Zoonosis Control, Hokkaido University, North-20, West-10, Kita-ku, Sapporo, 001-0020, Japan.
Hideaki HigashiDivision of Infection and Immunity, International Institute for Zoonosis Control, Hokkaido University, North-20, West-10, Kita-ku, Sapporo, 001-0020, Japan.
Hiroyuki YamaguchiDepartment of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Kita 12, Nishi 5, Kitaku, Sapporo, Hokkaido, 060-0812, Japan. hiroyuki@med.hokudai.ac.jp.ORCID http://orcid.org/0000-0003-3723-3361
Sapporo Medical University · JPHokkaido University · JPJuntendo University · JP

Funding

KAKENHI 16H05225KAKENHI 21H02726
6 · The paper itself

Abstract

The role of lymphocytes as a cornerstone of the inflammatory response in the invasive pathogenesis of Chlamydia trachomatis (Ct) LGV (L1-3) infection is unclear. Therefore, we assessed whether the adaptation of CtL2 to immortal lymphoid Jurkat cells under hypoxic conditions occurred through proinflammatory cytokine profile modification. The quantities of inclusion-forming units with chlamydial 16S rDNA confirmed that CtL2 grew well under hypoxic rather than normoxic conditions in the cells. Confocal microscopic imaging and transmission electron microscopy revealed the presence of bacterial progeny in the inclusions and showed that the inclusions were larger under hypoxic rather than normoxic conditions; this was supported by the results of 3D image construction. Furthermore, PCR-based analysis of proinflammatory cytokines revealed that the gene expression levels under hypoxic conditions were significantly higher than those under normoxic conditions. In particular, the expression of two genes (CXCL8 and CXCR3) was significantly diminished under normoxic conditions. Taken together, the results indicated that hypoxia promoted CtL2 growth in Jurkat cells while maintaining the levels of proinflammatory cytokines. Thus, Ct LGV infection in lymphocytes under hypoxic conditions might be crucial to a complete understanding of the invasive pathogenesis.

Indexed as

Chlamydia InfectionsChlamydia trachomatisCytokinesHumansHypoxiaJurkat CellsCytokines

Identifiers

PMID35859064
OpenAlexW4286209808

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.