Combining data acquisition modes in liquid-chromatography-tandem mass spectrometry for comprehensive determination of acylcarnitines in human serum.

D Luque-Córdoba, M Calderón-Santiago, F Priego-Capote

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Article in Metabolomics : Official journal of the Metabolomic Society, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

0.3field-weighted citation impact, top 45% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

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2 · The registry

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0 citing papers in PubMed, 4 citations in OpenAlex.

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5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

D Luque-CórdobaDepartment of Analytical Chemistry, University of Córdoba, Annex Marie Curie Building, Campus of Rabanales, Córdoba, Spain.

M Calderón-SantiagoDepartment of Analytical Chemistry, University of Córdoba, Annex Marie Curie Building, Campus of Rabanales, Córdoba, Spain.

F Priego-CapoteDepartment of Analytical Chemistry, University of Córdoba, Annex Marie Curie Building, Campus of Rabanales, Córdoba, Spain. feliciano.priego@uco.es.ORCID http://orcid.org/0000-0003-0697-719X

Instituto de Salud Carlos III · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Acylcarnitines (ACs) are metabolites involved in fatty acid β-oxidation and organic acid metabolism. Metabolic disorders associated to these two processes can be evaluated by determining the complete profile of ACs. In this research, we present an overall strategy for identification, confirmation, and quantitative determination of acylcarnitines in human serum. By this strategy we identified the presence of 47 ACs from C2 to C24 with detection of the unsaturation degree by application of a data-independent acquisition (DIA) liquid chromatography-tandem mass spectrometry (LC-MS/MS) method. Complementary, quantitative determination of ACs is based on a high-throughput and fully automated method consisting of solid-phase extraction on-line coupled to LC-MS/MS in data-dependent acquisition (DDA) to improve analytical features avoiding the errors associated to sample processing. Quantitation limits were at pg mL

Indexed as

MetabolomicsTandem Mass SpectrometryCarnitineChromatography, LiquidHumansacylcarnitineCarnitineAcylcarnitinesData-dependent acquisitionData-independent acquisitionMultiple reaction monitoringSerumSPE–LC–MS/MS

Identifiers

PMID35859020

DOI10.1186/s12944-021-01576-9

PMCPMC9300566

OpenAlexW4285992829

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.