ArticleJournal of applied microbiology2022
Evaluation of a novel multiplex qPCR method for rapid detection and quantification of pathogens associated with calf diarrhoea.
Article in Journal of applied microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Occurrence of respiratory and enteric bacteria and viruses in calves in Iceland.Acta veterinaria Scandinavica · 2026Article
- Development of a Multiplex PCR Assay for SelectedBiology · 2026Article
- Diversity ofVeterinary sciences · 2025Article
- Advances in diagnosis of diseases causing diarrhea in newborn calves.Veterinary research communications · 2025Review
- Establishment and epidemiological investigation of a dual fluorescent qPCR assay forFrontiers in cellular and infection microbiology · 2025Article
- Simultaneous detection of seven bacterial pathogens transmitted by flies using the reverse line blot hybridization assay.Parasites & vectors · 2024Article
- Evaluation of a novel multiplex qPCR method for rapid detection and quantification of pathogens associated with calf diarrhoea.Journal of applied microbiology · 2022Article
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Authors and funding
8 authors.
Funding
Abstract
aimsDiarrhoea is a common health problem in calves and a main reason for use of antimicrobials. It is associated with several bacterial, viral and parasitic pathogens, most of which are commonly present in healthy animals. Methods, which quantify the causative agents, may therefore improve confidence in associating a pathogen to the disease. This study evaluated a novel commercially available, multiplex quantitative polymerase chain reaction (qPCR) assay (Enterit4Calves) for detection and quantification of pathogens associated with calf-diarrhoea. METHODS AND
resultsPerformance of the method was first evaluated under laboratory conditions. Then it was compared with current routine methods for detection of pathogens in faecal samples from 65 calves with diarrhoea and in 30 spiked faecal samples. The qPCR efficiencies were between 84%-103% and detection limits of 100-1000 copies of nucleic acids per sample were observed. Correct identification was obtained on 42 strains of cultured target bacteria, with only one false positive reaction from 135 nontarget bacteria. Kappa values for agreement between the novel assay and current routine methods varied between 0.38 and 0.83.
conclusionThe novel qPCR method showed good performance under laboratory conditions and a fair to good agreement with current routine methods when used for testing of field samples. SIGNIFICANCE AND IMPACT OF STUDY: In addition to having fair to good detection abilities, the novel qPCR method allowed quantification of pathogens. In the future, use of quantification may improve diagnosis and hence treatment of calf diarrhoea.
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