ArticleActa parasitologica2022
Molecular Characterization of a New Nosema bombycis Strain Detected in Iranian Silkworm Farms.
Article in Acta parasitologica, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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Who cites it
1 citing paper in PubMed, 2 citations in OpenAlex.
- Diagnosis of Pebrine Disease in Silkworm Using Molecular Methods.Archives of Razi Institute · 2023Article
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7 authors at 3 institutions in 1 country.
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Abstract
purposePebrine as the most dangerous disease of silkworm mostly caused by Nosema species has caused huge economic losses. There is no information on the species and the genomic sequences of the pebrine-causing microsporidia in Iran.
methodsIn the present research, we tried to determine the sequences of two regions of rDNA using molecular methods. First, infected larvae and mother moths were collected from several farms in the north of Iran for identification and molecular characterization of microsporidian isolates. After extracting the spores and genomic DNA from the collected samples, two fragments of internal transcribed spacer (ITS) rDNA and small subunit (SSU) rDNA were amplified and sequenced, and registered in NCBI database and then, the phylogenetic tree was drawn.
resultsResults showed the obtained sequences (ITS rDNA: Accession No. MZ322002 and SSU rDNA: Accession No. MZ314703) represent a new strain of Nosema bombycis, which differs from the sequences deposited in the NCBI.
conclusionThe new N. bombycis strain identified in our study will help in control and management of the pebrine disease by specific detection of the infectious agent.
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