Evidence map›Paper›PMID 35857237›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Optogenetic Control of Human Stem Cell-Derived Neurons.

Rouhollah Habibey, Johannes Striebel, Kritika Sharma, Volker Busskamp

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Rouhollah HabibeyDepartment of Ophthalmology, Universitäts-Augenklinik Bonn, University of Bonn, Bonn, Germany.
Johannes StriebelDepartment of Ophthalmology, Universitäts-Augenklinik Bonn, University of Bonn, Bonn, Germany.
Kritika SharmaDepartment of Ophthalmology, Universitäts-Augenklinik Bonn, University of Bonn, Bonn, Germany.
Volker BusskampDepartment of Ophthalmology, Universitäts-Augenklinik Bonn, University of Bonn, Bonn, Germany. Volker.busskamp@uni-bonn.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Spontaneous and optogenetically evoked activities of human induced pluripotent stem cell (hiPSC)-derived neurons can be assessed by patch clamp and multi-electrode array (MEA) electrophysiology. Optogenetic activation of these human neurons facilitates the characterization of their functional properties at the single neuron and circuit level. Here we showcase the preparation of hiPSC-derived neurons expressing optogenetic actuators, in vitro optogenetic stimulation and simultaneous functional recordings using patch clamp and MEA electrophysiology.

Indexed as

Induced Pluripotent Stem CellsOptogeneticsAction PotentialsCell DifferentiationCells, CulturedHumansNeuronsBanker cultureInduced pluripotent stem cells (iPSCs)Long-term iPSC-derived neuronal cultureMulti-electrode array (MEA) electrophysiologyNeuron-astrocyte co-cultureOptogenetic stimulationPatch clamp

Identifiers

PMID35857237

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.