Evidence map›Paper›PMID 35857235›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Optogenetic Studies of Mitochondria.

Kai Chen, Patrick Ernst, Xiaoguang Margaret Liu, Lufang Zhou

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Kai ChenDepartment of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL, USA.
Patrick ErnstDepartment of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL, USA.
Xiaoguang Margaret LiuDepartment of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL, USA.
Lufang ZhouDepartment of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL, USA. lfzhou@uab.edu.
University of Alabama at Birmingham · US

Funding

Combine mitochondrial gene therapy and synthetic lethal chemotherapy to treat triple-negative breast cancerR01CA262028 · NCI · OHIO STATE UNIVERSITY · PI Xiaoguang Margaret Liu, Eddy Shih Hsin Yang · 2022 to 2026
$2.8M
Delineate the Pathophysiological Effect of Cardiomyocyte-specific Mitochondrial StressR01HL156581 · NHLBI · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI ZHOU, LUFANG · 2021 to 2025
$2.2M
Mitochondria-SR Redox Signaling and Ca2+ Handling in Healthy and Failing HeartsR01HL121206 · NHLBI · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI ZHOU, LUFANG · 2015 to 2019
$1.8M
NCI NIH HHS R01 CA262028NHLBI NIH HHS R01 HL121206NHLBI NIH HHS R01 HL156581
6 · The paper itself

Abstract

While optogenetic approaches have been widely used for remote control of cell membrane excitability and intracellular signaling pathways, their application in mitochondrial study has been limited, largely due to the challenge of effectively and specifically expressing heterologous light-gated rhodopsin channels in the mitochondria. Here, we describe the methods for expressing functional channelrhodopsin 2 (ChR2) proteins in the mitochondrial inner membrane with an unusually long mitochondrial leading sequence and characterizing optogenetic-mediated mitochondrial membrane potential (ΔΨ

Indexed as

MitochondriaOptogeneticsChannelrhodopsinsMitophagyRhodopsinChannelrhodopsinsRhodopsinApoptosisMitochondriaMitochondrial protein importMitophagyOptogeneticsPreconditioning

Identifiers

PMID35857235
PMCPMC10210090
OpenAlexW4286355456

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.