Evidence map›Paper›PMID 35857231›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Single-Cell Resolution Optogenetics Via Expression of Soma-Targeted Rhodopsins.

Changyang Linghu, I-Wen Chen, Dimitrii Tanese, Valeria Zampini, Or A Shemesh

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Changyang LinghuMcGovern Institute for Brain Research, MIT, Cambridge, MA, USA.
I-Wen ChenSorbonne Université, INSERM, CNRS, Institut de la Vision, Paris, France.
Dimitrii TaneseSorbonne Université, INSERM, CNRS, Institut de la Vision, Paris, France.
Valeria ZampiniSorbonne Université, INSERM, CNRS, Institut de la Vision, Paris, France.
Or A ShemeshMcGovern Institute for Brain Research, MIT, Cambridge, MA, USA. oshemesh@pitt.edu.

Funding

High speed, high precision volumetric multiphoton neural controlUF1NS107574 · NINDS · UNIVERSITY OF CALIFORNIA BERKELEY · PI ADESNIK, HILLEL · 2018 to 2018
$5.1M
NINDS NIH HHS UF1 NS107574
6 · The paper itself

Abstract

Optogenetics allows control of neural activity in genetically targeted neuron populations by light. Optogenetic control of individual neurons in neural circuits would enable powerful, causal investigations of neural connectivity and function at single-cell level and provide insights into how neural circuits operate. Such single-cell resolution optogenetics in neuron populations requires precise sculpting of light and subcellular targeting of optogenetic molecules. Here we describe a group of methods for single-cell resolution optogenetics in neuron cultures, in mouse brain slices, and in mouse cortex in-vivo, via patterned light and soma-targeted optogenetic molecules.

Indexed as

OptogeneticsRhodopsinAnimalsCell BodyMiceNeuronsRhodopsinCell-body-targeted opsinsComputer-generated holographyFunctional connectivitySingle-cell controlSingle-cell optogeneticsSomatic optogeneticsSynaptic transmissionTwo-photon stimulation

Identifiers

PMID35857231

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.