Evidence map›Paper›PMID 35854639›Full record

ArticleMedical science monitor : international medical journal of experimental and clinical research2022

Bioinformatics Profiling and Experimental Validation of 4 Differentially-Expressed LIM Genes in the Course of Colorectal-Adenoma-Carcinoma.

Ying He, Zongfu Pan, Qian Shi, Xilin Zhang, Weiyun Shen, Lixia Huo, Huihui Guo, Chengwu Tang, Yuhang Ling

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Medical science monitor : international medical journal of experimental and clinical research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Ying HeCentral Laboratory, The First Affliated Hospital of Huzhou University, Huzhou, Zhejiang, China (mainland).
Zongfu PanDepartment of Pharmacy, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, China (mainland).
Qian ShiCentral Laboratory, The First Affliated Hospital of Huzhou University, Huzhou, Zhejiang, China (mainland).
Xilin ZhangCentral Laboratory, The First Affliated Hospital of Huzhou University, Huzhou, Zhejiang, China (mainland).
Weiyun ShenCentral Laboratory, The First Affliated Hospital of Huzhou University, Huzhou, Zhejiang, China (mainland).
Lixia HuoCentral Laboratory, The First Affliated Hospital of Huzhou University, Huzhou, Zhejiang, China (mainland).
Huihui GuoCentral Laboratory, The First Affliated Hospital of Huzhou University, Huzhou, Zhejiang, China (mainland).
Chengwu TangCentral Laboratory, The First Affliated Hospital of Huzhou University, Huzhou, Zhejiang, China (mainland).
Yuhang LingCentral Laboratory, The First Affliated Hospital of Huzhou University, Huzhou, Zhejiang, China (mainland).
Huzhou University · CNHangzhou Medical College · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND LIM domain proteins play crucial roles in tumors by interacting with diverse proteins. However, their roles in the course of colorectal mucosa-adenoma-carcinoma remain unclear. This study aimed to depict their dynamic expression profiles and elucidate their potential functions in this transition course. MATERIAL AND METHODS Differentially-expressed LIM proteins (DELGs) in paired adenomas, carcinomas, and mucosae were identified using the GEO dataset (GSE 117606) and validated by immunohistochemistry using our tissue microarray. Kaplan-Meier survival analysis, WGCNA, module-trait analysis, and KEGG enrichment were conducted. The correlation of DELGs expression levels with immune infiltration was assessed using the ESTIMATE package and TISCH database. The role of DELGs of interest was validated using cell proliferation, migration, and invasion assays. RESULTS Four DELGs were identified - LMO3, FHL1, NEBL, and TGFB1I1 - all of which were of significance in prognosis. Module-trait correlation and KEGG enrichment revealed their involvement in cancer-related signaling. Immunohistochemistry showed gradual downregulation of LMO3 but upregulation of NEBL in the mucosa-adenoma-carcinoma sequence. The opposite expression patterns were observed for FHL1 and TGFB1I1 in tumor epithelium and mesenchyme. High expression levels of the DELGs were correlated with increased infiltration of NK, NKT, and macrophages, except for NEBL. Importantly, LMO3 inhibited proliferation, migration, and invasion of colon epithelial cells. CONCLUSIONS This study identified 4 differentially-expressed LIM genes - LMO3, FHL1, TGFB1I1, and NEBL - and revealed they were involved in the mucosa-adenoma-carcinoma sequence via regulating cancer-related pathways, influencing epigenetic field, or affecting immune infiltration. Our findings provide new insights into the roles of LIM proteins in the course of mucosa-adenoma-carcinoma.

Indexed as

AdenomaColorectal NeoplasmsLIM Domain ProteinsAdaptor Proteins, Signal TransducingCarrier ProteinsComputational BiologyCytoskeletal ProteinsHumansIntracellular Signaling Peptides and ProteinsMuscle ProteinsAdaptor Proteins, Signal TransducingCarrier ProteinsCytoskeletal ProteinsFHL1 protein, humanIntracellular Signaling Peptides and ProteinsLIM Domain ProteinsLMO3 protein, humanMuscle ProteinsNEBL protein, humanTGFB1I1 protein, human

Identifiers

PMID35854639
PMCPMC9310551
OpenAlexW4285728829

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.