ReviewEvoDevo2022
The red flour beetle T. castaneum: elaborate genetic toolkit and unbiased large scale RNAi screening to study insect biology and evolution.
Review in EvoDevo, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 58 citations in OpenAlex.
- Sixteen isotropic 3D fluorescence live-imaging datasets of gastrulation in the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae).Scientific data · 2026Article
- BeetleAtlas 2: An enhanced Tribolium castaneum web resource for tissue and developmental transcriptomics allowing refinement of gene predictions.PLoS computational biology · 2026Article
- Article
- Development and characterization of novel microsatellite markers for Tribolium castaneum (Coleoptera: Tenebrionidae).Journal of insect science (Online) · 2026Article
- Defining the breakpoints of the vmicroPublication biology · 2026Article
- CRISPR screening reveals genetic regulators associated with the evolution of eye degeneration.bioRxiv : the preprint server for biology · 2025Article
- Orthologous genes of the red flour beetle Tribolium castaneum and the vinegar fly Drosophila melanogaster.BMC genomic data · 2025Article
- Odorant Binding Proteins inInsects · 2025Review
- Article
- NCBI Orthologs: Public Resource and Scalable Method for Computing High-Precision Orthologs Across Eukaryotic Genomes.Journal of molecular evolution · 2025Article
- Genomic exploration of Bemisia tabaci microRNAs using predictive modeling and confirmation through experimental evidence.Scientific reports · 2025Article
- The quest for the best target genes for RNAi-mediated pest control.Insect molecular biology · 2025Review
- Article
- The relationship between taxonomic classification and applied entomology: stored product pests as a model group.Journal of insect science (Online) · 2025Review
- Article
- Superior target genes and pathways for RNAi-mediated pest control revealed by genome-wide analysis in the beetle Tribolium castaneum.Pest management science · 2025Article
- Long-read genome assembly of the insect model organismGenome research · 2024Article
- Generating and testing the efficacy of reagents for CRISPR/Cas9 homology directed repair-based manipulations in Tribolium.Journal of insect science (Online) · 2024Article
- Temporal control of RNAi reveals both robust and labile feedback loops in the segmentation clock of the red flour beetle.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A life-history allele of large effect shortens developmental time in a wild insect population.Nature ecology & evolution · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The red flour beetle Tribolium castaneum has emerged as an important insect model system for a variety of topics. With respect to studying gene function, it is second only to the vinegar fly D. melanogaster. The RNAi response in T. castaneum is exceptionally strong and systemic, and it appears to target all cell types and processes. Uniquely for emerging model organisms, T. castaneum offers the opportunity of performing time- and cost-efficient large-scale RNAi screening, based on commercially available dsRNAs targeting all genes, which are simply injected into the body cavity. Well established transgenic and genome editing approaches are met by ease of husbandry and a relatively short generation time. Consequently, a number of transgenic tools like UAS/Gal4, Cre/Lox, imaging lines and enhancer trap lines are already available. T. castaneum has been a genetic experimental system for decades and now has become a workhorse for molecular and reverse genetics as well as in vivo imaging. Many aspects of development and general biology are more insect-typical in this beetle compared to D. melanogaster. Thus, studying beetle orthologs of well-described fly genes has allowed macro-evolutionary comparisons in developmental processes such as axis formation, body segmentation, and appendage, head and brain development. Transgenic approaches have opened new ways for in vivo imaging. Moreover, this emerging model system is the first choice for research on processes that are not represented in the fly, or are difficult to study there, e.g. extraembryonic tissues, cryptonephridial organs, stink gland function, or dsRNA-based pesticides.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.