Evidence map›Paper›PMID 35854245›Full record

ArticleBMC cancer2022

circ_0000045 promotes proliferation, migration, and invasion of head and neck squamous cell carcinomas via regulating HSP70 and MAPK pathway.

Ronghao Sun, Yuqiu Zhou, Yongcong Cai, Chunyan Shui, Xu Wang, Jingqiang Zhu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC cancer, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.4field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Ronghao SunDepartment of Head and Neck Surgery, Sichuan Cancer Hospital and Institute,University of Electronic Science and Technology of China, No.55, 4th section of Southern Renmin Road, Chengdu, Sichuan, 610041, China. sunronghao666@163.com.
Yuqiu ZhouDepartment of Head and Neck Surgery, Sichuan Cancer Hospital and Institute,University of Electronic Science and Technology of China, No.55, 4th section of Southern Renmin Road, Chengdu, Sichuan, 610041, China.
Yongcong CaiDepartment of Head and Neck Surgery, Sichuan Cancer Hospital and Institute,University of Electronic Science and Technology of China, No.55, 4th section of Southern Renmin Road, Chengdu, Sichuan, 610041, China.
Chunyan ShuiDepartment of Head and Neck Surgery, Sichuan Cancer Hospital and Institute,University of Electronic Science and Technology of China, No.55, 4th section of Southern Renmin Road, Chengdu, Sichuan, 610041, China.
Xu WangDepartment of Head and Neck Surgery, Sichuan Cancer Hospital and Institute,University of Electronic Science and Technology of China, No.55, 4th section of Southern Renmin Road, Chengdu, Sichuan, 610041, China.
Jingqiang ZhuDepartment of Head and Neck Surgery, Sichuan Cancer Hospital and Institute,University of Electronic Science and Technology of China, No.55, 4th section of Southern Renmin Road, Chengdu, Sichuan, 610041, China. zjq-wkys@163.com.
Sichuan Cancer Hospital · CNUniversity of Electronic Science and Technology of China · CNSichuan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveHead and neck squamous cell carcinoma (HNSCC) is one severe malignancy driven by complex cellular and signaling mechanisms. However, the roles of circular RNAs (circRNAs) in HNSCC's development remains poorly understood. Therefore, this study investigated the functions of differentially expressed circRNAs in regulating HNSCC cell functions.

methodsDifferentially expressed circRNAs were characterized through RNA sequencing in HNSCC tissues. CircRNA's identity was then confirmed using RT-PCR and Sanger's sequencing. Next, expression levels of circRNA and mRNA were detected by qRT-PCR, after which protein abundances were measured by Western blotting. Subsequently, the proliferation, migration, and invasion of HNSCC cells was assessed by MTS, wound healing, and Transwell system, respectively, followed by identification of circRNA-binding proteins in HNSCC cells by circRNA pull-down, coupled with mass spectrometry.

resultsGreat alterations in circRNA profiles were detected in HNSCC tissues, including the elevated expression of circ_0000045. As observed, silencing of circ_0000045 effectively repressed the proliferation, migration, and invasion of HNSCC cell lines (FaDu and SCC-9). Contrarily, circ_0000045's overexpression promoted the proliferation, migration, and invasion in FaDu and SCC-9 cells. Results also showed that circ_0000045 was associated with multiple RNA-binding proteins in HNSCC cells, such as HSP70. Moreover, circ_0000045 knockdown enhanced HSP70 expression and inhibited JNK2 and P38's expression in HNSCC cells, which were oppositely regulated by circ_0000045's overexpression.

conclusionThe high expression of circ_0000045; therefore, promoted cell proliferation, migration, and invasion during HNSCC's development through regulating HSP70 protein and mitogen-activated protein kinase signaling.

Indexed as

Head and Neck NeoplasmsMicroRNAsCell Line, TumorCell MovementCell ProliferationHumansRNA, CircularSquamous Cell Carcinoma of Head and NeckMicroRNAsRNA, Circularcirc_0000045circRNAHNSCCHSP70JNK2MAPKP38

Identifiers

PMID35854245
PMCPMC9297571
OpenAlexW4285891424

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.