Evidence map›Paper›PMID 35853030›Full record

ArticlePloS one2022

Evaluation of different combination of pam2CSK4, poly (I:C) and imiquimod enhance immune responses to H9N2 avian influenza antigen in dendritic cells and duck.

Aiguo Zhang, Deyin Li, Chao Song, Huiyuan Jing, Hongfei Li, Junxian Mi, Guizhi Zhang, Shuangxing Jin, Xiaoli Ren, Heping Huangfu and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Aiguo ZhangCollege of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China.ORCID 0000-0003-1059-6410
Deyin LiCollege of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China.
Chao SongCollege of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China.
Huiyuan JingCollege of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China.
Hongfei LiCollege of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China.
Junxian MiCollege of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China.
Guizhi ZhangCollege of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China.
Shuangxing JinCollege of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China.
Xiaoli RenCollege of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China.
Heping HuangfuCollege of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China.
Dongmei ShiCollege of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China.
Ruiai ChenHuannong (Zhaoqing) Institute of Biotechnology Co. Ltd., Zhaoqing, Guangdong, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Current commercial H9 avian influenza viruses (AIVs) vaccines cannot provide satisfactory antibody titers and protective immunity against AIVs in duck. Toll like receptors (TLR) ligand as AIVs adjuvants can activate dendritic cells to improve immune responses in multiple animals, while the studies were absent in duck. Therefore, we investigated TLR ligands pam2CSK4, poly (I:C) and/or imiquimod enhance immune responses to inactivated H9N2 avian influenza antigen (H9N2 IAIV) in peripheral blood monocyte-derived dendritic cells (MoDCs) and duck. In vitro, we observed that transcription factor NF-κB, Th1/Th2 type cytokines (IFN-γ, IL-2 and IL-6) and the ability of catching H9N2 IAIV antigen were significantly up-regulated when H9N2 IAIV along with TLR ligands (pam2CSK4, poly (I:C) and imiquimod, alone or combination) in duck MoDCs. Also, the best enhancement effects were showed in combination of pam2CSK4, poly (I:C) and imiquimod group, whereas IFN-α showed no significant enhancement in all experimental groups. In vivo, the results demonstrated that the percentages of CD4+/ CD8+ T lymphocytes, the levels of Th1/Th2 type cytokines and H9N2 HI titers were significant enhanced in combination of pam2CSK4, poly (I:C) and imiquimod group. However, pam2CSK4 alone or combining with imiquimod showed no enhancement or additive effects on Th1 cytokines (IFN-γ and IL-2), Th2 cytokines (IL-6) and HI titers in Muscovy duck, respectively. Taken together, our results concluded that not all TLR ligands showed enhancement of immune responses to H9N2 IAIV in duck. The combination of poly (I:C), imiquimod and pam2CSK4 that can be an effectively adjuvant candidate for H9N2 AIVs inactivated vaccine in duck, which provide novel insights in explore waterfowl vaccine.

Indexed as

Influenza A Virus, H9N2 SubtypeInfluenza, HumanInfluenza in BirdsInfluenza VaccinesAdjuvants, ImmunologicAnimalsChickensCytokinesDendritic CellsDucksHumansImiquimodImmunityInterleukin-2Interleukin-6OligopeptidesAdjuvants, ImmunologicCytokinesImiquimodInfluenza VaccinesInterleukin-2Interleukin-6OligopeptidesPAM2-CSK4Poly I-CToll-Like Receptor 2Toll-Like Receptor 9Toll-Like Receptors

Identifiers

PMID35853030
PMCPMC9295992

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.