ArticleAnalytical chemistry2022
Label-Free Imaging of Single Proteins and Binding Kinetics Using Total Internal Reflection-Based Evanescent Scattering Microscopy.
Article in Analytical chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Meta-surface plasmon resonance microscopy for sensitive, high-throughput, and long-term label-free analysis of cell adhesion dynamics.Journal of nanobiotechnology · 2025Article
- The Emergence of Nanofluidics for Single-Biomolecule Manipulation and Sensing.Analytical chemistry · 2025Review
- Review
- Dielectric Surface-Based Biosensors for Enhanced Detection of Biomolecular Interactions: Advances and Applications.Biosensors · 2024Review
- Molecular fingerprinting of biological nanoparticles with a label-free optofluidic platform.Nature communications · 2024Article
- Label-Free Optical Imaging of Nanoscale Single Entities.ACS sensors · 2024Review
- Label-Free Tracking of Proteins through Plasmon-Enhanced Interference.ACS nanoscience Au · 2024Article
- Molecular fingerprinting of biological nanoparticles with a label-free optofluidic platform.Research square · 2023Article
- Label-free quantification of protein binding to lipid vesicles using transparent waveguide evanescent-field scattering microscopy with liquid control.Biomedical optics express · 2023Article
- Plasmonic Scattering Microscopy for Label-Free Imaging of Molecular Binding Kinetics: From Single Molecules to Single Cells.Chemistry methods : new approaches to solving problems in chemistry · 2023Article
- Label-free imaging and biomarker analysis of exosomes with plasmonic scattering microscopy.Chemical science · 2022Article
- Single-Protein Identification by Simultaneous Size and Charge Imaging Using Evanescent Scattering Microscopy.ACS sensors · 2022Article
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Authors and funding
8 authors.
Funding
Abstract
Single-molecule detection can push beyond ensemble averages and reveal the statistical distributions of molecular properties. Measuring the binding kinetics of single proteins also represents one of the critical and challenging tasks in protein analysis. Here, we report total internal reflection-based evanescent scattering microscopy with label-free single-protein detection capability. Total internal reflection is employed to excite the evanescent field to enhance light-analyte interaction and reduce environmental noise. As a result, the system provides wide-field imaging capability and allows excitation and observation using one objective. In addition, this system quantifies protein binding kinetics by simultaneously counting the binding of individual molecules and recording their binding sites with nanometer precision, providing a digital method to measure binding kinetics with high spatiotemporal resolution. This approach does not employ specially designed microspheres or nanomaterials and may pave a way for label-free single-protein analysis in conventional microscopy.
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