Evidence map›Paper›PMID 35849973›Full record

ArticleJournal of magnetic resonance (San Diego, Calif. : 1997)2022

NMR as a readout to monitor and restore the integrity of complex chemoenzymatic reactions.

Kenneth A Marincin, Yousang Hwang, Everett S Kengmana, David J Meyers, Dominique P Frueh

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of magnetic resonance (San Diego, Calif. : 1997), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 93% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Kenneth A MarincinDepartment of Biophysics and Biophysical Chemistry, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
Yousang HwangDepartment of Pharmacology and Molecular Sciences Synthetic Core Facility, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
Everett S KengmanaDepartment of Chemical and Biomolecular Engineering, Johns Hopkins Whiting School of Engineering, Baltimore, MD 21218, USA.
David J MeyersDepartment of Pharmacology and Molecular Sciences Synthetic Core Facility, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.
Dominique P FruehDepartment of Biophysics and Biophysical Chemistry, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA. Electronic address: dfrueh1@jhmi.edu.
Johns Hopkins University · US

Funding

QAQC Johns Hopkins Institute for Clinical and Translational ResearchUL1TR003098 · NCATS · JOHNS HOPKINS UNIVERSITY · PI FORD, DANIEL ERNEST · 2019 to 2023
$57.7M
The Chemistry-Biology Interface Program at Johns Hopkins UniversityT32GM080189 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI ROKITA, STEVEN E · 2008 to 2022
$3.5M
NMR studies of heterocyclization and epimerization in yersiniabactin synthesisR01GM104257 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI FRUEH, DOMINIQUE PASCAL · 2013 to 2023
$3.0M
The Chemistry-Biology Interface Program at Johns Hopkins UniversityT32GM149382 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI STEVEN E ROKITA · 2023 to 2026
$1.4M
NCATS NIH HHS UL1 TR003098NIGMS NIH HHS R01 GM104257NIGMS NIH HHS T32 GM080189NIGMS NIH HHS T32 GM149382
6 · The paper itself

Abstract

The non-invasive nature of NMR offers a means to monitor biochemical reactions in situ at the atomic level. We harness this advantage to monitor a complex chemoenzymatic reaction that sequentially modifies reagents and loads the product on a nonribosomal peptide synthetase carrier protein. We present a protocol including a pulse sequence that permits to assess both the integrity of reagents and the completion of each step in the reaction, thus alleviating otherwise time-consuming and costly approaches to debug and repeat inefficient reactions. This study highlights the importance of NMR as a tool to establish reliable and reproducible experimental conditions in biochemical studies.

Indexed as

Magnetic Resonance ImagingMagnetic Resonance SpectroscopyChemoenzymatic reactionsDiffusionIsotope filterNMR

Identifiers

PMID35849973
PMCPMC9463103
OpenAlexW4284881971

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.