Evidence map›Paper›PMID 35849625›Full record

ArticlePLoS genetics2022

Structural insights into Pot1-ssDNA, Pot1-Tpz1 and Tpz1-Ccq1 Interactions within fission yeast shelterin complex.

Hong Sun, Zhenfang Wu, Yuanze Zhou, Yanjia Lu, Huaisheng Lu, Hongwen Chen, Shaohua Shi, Zhixiong Zeng, Jian Wu, Ming Lei

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PLoS genetics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. NAR molecular medicine · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Hong SunState Key Laboratory of Molecular Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China.
Zhenfang WuNinth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.ORCID 0000-0002-7100-8849
Yuanze ZhouNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China.
Yanjia LuNinth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Huaisheng LuNinth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Hongwen ChenState Key Laboratory of Molecular Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China.
Shaohua ShiNinth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Zhixiong ZengShandong Provincial Key Laboratory of Microbial Engineering, College of Bioengineering, Qilu University of Technology, Shandong, China.
Jian WuNinth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Ming LeiNinth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.ORCID 0000-0002-1153-4791
Shanghai Jiao Tong University · CNChinese Academy of Sciences · CNHuazhong Agricultural University · CNQilu University of Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The conserved shelterin complex caps chromosome ends to protect telomeres and regulate telomere replication. In fission yeast Schizosaccharomyces pombe, shelterin consists of telomeric single- and double-stranded DNA-binding modules Pot1-Tpz1 and Taz1-Rap1 connected by Poz1, and a specific component Ccq1. While individual structures of the two DNA-binding OB folds of Pot1 (Pot1OB1-GGTTAC and Pot1OB2-GGTTACGGT) are available, structural insight into recognition of telomeric repeats with spacers by the complete DNA-binding domain (Pot1DBD) remains an open question. Moreover, structural information about the Tpz1-Ccq1 interaction requires to be revealed for understanding how the specific component Ccq1 of S. pombe shelterin is recruited to telomeres to function as an interacting hub. Here, we report the crystal structures of Pot1DBD-single-stranded-DNA, Pot1372-555-Tpz1185-212 and Tpz1425-470-Ccq1123-439 complexes and propose an integrated model depicting the assembly mechanism of the shelterin complex at telomeres. The structure of Pot1DBD-DNA unveils how Pot1 recognizes S. pombe degenerate telomeric sequences. Our analyses of Tpz1-Ccq1 reveal structural basis for the essential role of the Tpz1-Ccq1 interaction in telomere recruitment of Ccq1 that is required for telomere maintenance and telomeric heterochromatin formation. Overall, our findings provide valuable structural information regarding interactions within fission yeast shelterin complex at 3' ss telomeric overhang.

Indexed as

SchizosaccharomycesSchizosaccharomyces pombe ProteinsTelomeraseCarrier ProteinsDNA, Single-StrandedProtein BindingShelterin ComplexTelomereTelomere-Binding ProteinsCarrier ProteinsCcq1 protein, S pombeDNA, Single-Strandedpot1 protein, S pombePoz1 protein, S pombeRap1 protein, S pombeSchizosaccharomyces pombe ProteinsShelterin Complextaz1 protein, S pombeTelomeraseTelomere-Binding Proteins

Identifiers

PMID35849625
PMCPMC9333443
OpenAlexW4285728082

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.