Evidence map›Paper›PMID 35849211›Full record

ArticleArchives of microbiology2022

Cloning, expression and characterization of PURase gene from Pseudomonas sp. AKS31.

Mousumi Saha, Shankari Prasad Dutta, Goutam Mukherjee, Aparajita Basu, Debarati Majumder, Alok Kumar Sil

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In one paragraph

Article in Archives of microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Mousumi SahaDepartment of Microbiology, Ballygunge Science College, University of Calcutta, 35, Ballygunge Circular Road, Calcutta, West Bengal, 700019, India.
Shankari Prasad DuttaDepartment of Biochemistry, Bose Institute, P 1/12 CIT Road Scheme VII M, Calcutta, West Bengal, 700054, India.
Goutam MukherjeeDepartment of Microbiology, Ballygunge Science College, University of Calcutta, 35, Ballygunge Circular Road, Calcutta, West Bengal, 700019, India.
Aparajita BasuDepartment of Microbiology, Ballygunge Science College, University of Calcutta, 35, Ballygunge Circular Road, Calcutta, West Bengal, 700019, India.
Debarati MajumderDepartment of Microbiology, Ballygunge Science College, University of Calcutta, 35, Ballygunge Circular Road, Calcutta, West Bengal, 700019, India.
Alok Kumar SilDepartment of Microbiology, Ballygunge Science College, University of Calcutta, 35, Ballygunge Circular Road, Calcutta, West Bengal, 700019, India. alokksil7@gmail.com.ORCID http://orcid.org/0000-0001-8817-3376
University of Calcutta · INBose Institute · IN

Funding

Science and Engineering Research Board PDF/2017/000854
6 · The paper itself

Abstract

Polyurethane (PUR) is a soil and aquatic contaminant throughout the world. Towards bioremediation, in a previous study, a soil bacterium, Pseudomonas sp. AKS31, capable of efficiently degrading PUR was isolated. Polyurethanase (PURase) enzyme is capable of cleaving the ester bond of PUR and is considered as a key regulator of PUR biodegradation. Hence, for a high yield, easy purification, and further characterization, the aim of this study was to clone and overexpress the PURase gene of this isolate. The current study also investigated structural aspects of this enzyme through predictive bioinformatics analyses. In this context, the PURase gene of the isolate was cloned and expressed in E. coli using pET28(a)

Indexed as

Escherichia coliPseudomonasBiodegradation, EnvironmentalCloning, MolecularLipasePolyurethanesSoilLipasePolyurethanesSoilBioremediationComputational analysisPolyurethanasePolyurethanePseudomonas

Identifiers

PMID35849211
OpenAlexW4285801870

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.