ArticleeLife2022
Deconstructing cold-induced brown adipocyte neogenesis in mice.
Article in eLife, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 1 synthesis or guideline pooled it, 51 citations in OpenAlex.
- Towards a consensus atlas of human and mouse adipose tissue at single-cell resolution.Nature metabolism · 2025Guideline
- The perineurium integrates leptin with its sympathetic outflow to protect against obesity.Nature metabolism · 2026Article
- Age-dependent progenitor switching shapes adult brown adipose tissue heterogeneity.Research square · 2026Article
- Spatial Analysis of Perivascular Adipose Tissue Gene Expression with SABER-FISH.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Adipose Progenitor Cells in Thermogenesis and Metabolic Regulation.Physiology (Bethesda, Md.) · 2026Review
- The role of immune responses and microbiota in adipose tissue homeostasis.The Journal of physiology · 2025Review
- Single-nucleus mRNA-sequencing reveals dynamics of lipogenic and thermogenic adipocyte populations in murine brown adipose tissue in response to cold exposure.Molecular metabolism · 2025Article
- Spatial Transcriptomics of Adipose Tissue: Technologies, Applications, and Challenges.Journal of obesity & metabolic syndrome · 2025Review
- Regulatory T cells in brown adipose tissue safeguard thermogenesis by restraining interferon-γ-producing lymphocytes.Science immunology · 2025Article
- Cold exposure stimulates cross-tissue metabolic rewiring to fuel glucose-dependent thermogenesis in brown adipose tissue.Science advances · 2025Article
- PVAT adipocyte: energizing, modulating, and structuring vascular function during normotensive and hypertensive states.American journal of physiology. Heart and circulatory physiology · 2025Review
- MACanalyzeR scRNAseq analysis tool reveals PPARγNature communications · 2025Article
- Article
- G-Protein-Coupled Receptor (GPCR) Signaling and Pharmacology in Metabolism: Physiology, Mechanisms, and Therapeutic Potential.Biomolecules · 2025Review
- Single-nucleus analysis of thoracic perivascular adipose tissue reveals critical changes in cell composition, communication, and gene regulatory networks induced by a high fat hypertensive diet.bioRxiv : the preprint server for biology · 2025Article
- Immune Regulatory Crosstalk in Adipose Tissue Thermogenesis.Comprehensive Physiology · 2025Review
- Diverse Functions of Macrophages in Obesity and Metabolic Dysfunction-Associated Steatotic Liver Disease: Bridging Inflammation and Metabolism.Immune network · 2025Review
- Article
- Decoding temporal thermogenesis: coregulator selectivity and transcriptional control in brown and beige adipocytes.Adipocyte · 2024Article
- Single-Cell Landscape and a Macrophage Subset Enhancing Brown Adipocyte Function in Diabetes.Diabetes & metabolism journal · 2024Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Cold exposure triggers neogenesis in classic interscapular brown adipose tissue (iBAT) that involves activation of β1-adrenergic receptors, proliferation of PDGFRA+ adipose tissue stromal cells (ASCs), and recruitment of immune cells whose phenotypes are presently unknown. Single-cell RNA-sequencing (scRNA-seq) in mice identified three ASC subpopulations that occupied distinct tissue locations. Of these, interstitial ASC1 were found to be direct precursors of new brown adipocytes (BAs). Surprisingly, knockout of β1-adrenergic receptors in ASCs did not prevent cold-induced neogenesis, whereas pharmacological activation of the β3-adrenergic receptor on BAs was sufficient, suggesting that signals derived from mature BAs indirectly trigger ASC proliferation and differentiation. In this regard, cold exposure induced the delayed appearance of multiple macrophage and dendritic cell populations whose recruitment strongly correlated with the onset and magnitude of neogenesis across diverse experimental conditions. High-resolution immunofluorescence and single-molecule fluorescence in situ hybridization demonstrated that cold-induced neogenesis involves dynamic interactions between ASC1 and recruited immune cells that occur on the micrometer scale in distinct tissue regions. Our results indicate that neogenesis is not a reflexive response of progenitors to β-adrenergic signaling, but rather is a complex adaptive response to elevated metabolic demand within brown adipocytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.