ArticleACS photonics2022
High Resolution Fluorescence Lifetime Maps from Minimal Photon Counts.
Article in ACS photonics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The trial behind it
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Who cites it
13 citing papers in PubMed.
- Re-establishment of TAD boundary organization during DNA replication.bioRxiv : the preprint server for biology · 2026Article
- Resolving fluorescently labeled species using highly multiplexed spectral FLIM.Scientific reports · 2026Article
- Chemical fluorophores for fluorescence lifetime imaging.Chemical Society reviews · 2026Review
- Fluorescence Lifetime Imaging Microscopy Analysis of Isolated Melanosomes.Chemphyschem : a European journal of chemical physics and physical chemistry · 2025Article
- Simultaneous particle tracking, phase retrieval and point spread function reconstruction.bioRxiv : the preprint server for biology · 2025Article
- Building Fluorescence Lifetime Maps Photon-by-Photon by Leveraging Spatial Correlations.ACS photonics · 2023Article
- Article
- Fluorescence lifetime tracking and imaging of single moving particles assisted by a low-photon-count analysis algorithm.Biomedical optics express · 2023Article
- Single-photon smFRET. I: Theory and conceptual basis.Biophysical reports · 2023Article
- Fluorescence lifetime: Beating the IRF and interpulse window.Biophysical journal · 2023Article
- Resolving Molecular Heterogeneity with Single-Molecule Centrifugation.Journal of the American Chemical Society · 2023Article
- Single-photon smFRET. III. Application to pulsed illumination.Biophysical reports · 2022Article
- High-precision estimation of emitter positions using Bayesian grouping of localizations.Nature communications · 2022Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Fluorescence lifetime imaging microscopy (FLIM) may reveal subcellular spatial lifetime maps of key molecular species. Yet, such a quantitative picture of life necessarily demands high photon budgets at every pixel under the current analysis paradigm, thereby increasing acquisition time and photodamage to the sample. Motivated by recent developments in computational statistics, we provide a direct means to update our knowledge of the lifetime maps of species of different lifetimes from direct photon arrivals, while accounting for experimental features such as arbitrary forms of the instrument response function (IRF) and exploiting information from empty laser pulses not resulting in photon detection. Our ability to construct lifetime maps holds for arbitrary lifetimes, from short lifetimes (comparable to the IRF) to lifetimes exceeding interpulse times. As our method is highly data efficient, for the same amount of data normally used to determine lifetimes and photon ratios, working within the Bayesian paradigm, we report direct blind unmixing of lifetimes with subnanosecond resolution and subpixel spatial resolution using standard raster scan FLIM images. We demonstrate our method using a wide range of simulated and experimental data.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.