Evidence map›Paper›PMID 35846350›Full record

ArticleFrontiers in cell and developmental biology2022

Design and Initial Characterization of a Small Near-Infrared Fluorescent Calcium Indicator.

Mikhail E Matlashov, Jorge Vera, Ludmila A Kasatkina, Kamran Khodakhah, Vladislav V Verkhusha

Open access · goldAbstract read
In one paragraph

Article in Frontiers in cell and developmental biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Calcium imaging: a technique to monitor calcium dynamics in biological systems.Physiology and molecular biology of plants : an international journal of functional plant biology · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 2 countries.

Mikhail E MatlashovDepartment of Genetics and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY, United States.
Jorge VeraDepartment of Neuroscience, Albert Einstein College of Medicine, Bronx, NY, United States.
Ludmila A KasatkinaDepartment of Genetics and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY, United States.
Kamran KhodakhahDepartment of Neuroscience, Albert Einstein College of Medicine, Bronx, NY, United States.
Vladislav V VerkhushaDepartment of Genetics and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY, United States.
Albert Einstein College of Medicine · US

Funding

Near-Infrared Fluorescent Proteins, Biosensors and Optogenetic ToolsR35GM122567 · NIGMS · ALBERT EINSTEIN COLLEGE OF MEDICINE, INC · PI Vladislav Verkhusha · 2017 to 2026
$4.3M
Cerebellum and AddictionR01DA044761 · NIDA · ALBERT EINSTEIN COLLEGE OF MEDICINE, INC · PI KHODAKHAH, KAMRAN · 2017 to 2021
$3.6M
Cerebellum and Mental DisordersR01MH115604 · NIMH · ALBERT EINSTEIN COLLEGE OF MEDICINE, INC · PI KHODAKHAH, KAMRAN · 2018 to 2022
$2.9M
NIDA NIH HHS R01 DA044761NIGMS NIH HHS R35 GM122567NIMH NIH HHS R01 MH115604
6 · The paper itself

Abstract

Near-infrared (NIR) genetically encoded calcium indicators (GECIs) are becoming powerful tools for neuroscience. Because of their spectral characteristics, the use of NIR GECIs helps to avoid signal loss from the absorption by body pigments, light-scattering, and autofluorescence in mammalian tissues. In addition, NIR GECIs do not suffer from cross-excitation artifacts when used with common fluorescent indicators and optogenetics actuators. Although several NIR GECIs have been developed, there is no NIR GECI currently available that would combine the high brightness in cells and photostability with small size and fast response kinetics. Here, we report a small FRET-based NIR fluorescent calcium indicator iGECInano. We characterize iGECInano

Indexed as

biosensorcalcium imagingfar-redFRETGECIiRFP

Identifiers

PMID35846350
PMCPMC9277108
OpenAlexW4283688652

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.